Mouse Hsp70 family ATPase SSC1 Monoclonal Antibody | anti-SSC1 antibody

Hsp70 family ATPase SSC1 Mouse mAb for Yeast

Cat. Num. AAA435449

• Gene Names: SSC1; ENS1
• Reactivity: Saccharomyces Cerevisiae
• Applications: Western Blot
• Synonyms: Hsp70 family ATPase SSC1; Monoclonal Antibody; Hsp70 family ATPase SSC1 Mouse mAb for Yeast; SSC1 Mouse mAb; anti-SSC1 antibody

• Host: Mouse
• Reactivity: Saccharomyces Cerevisiae
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 654

• Applicable Applications for anti-SSC1 antibody: Western Blot (WB)
• Application Notes: WB 1:1000 - 1:5000; ; IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Source Note: This monoclonal antibody is produced by immunizing mice with a polypeptide corresponding to hsp70 family ATPase SSC1 protein.
• Preparation and Storage: Store at -20°C. Stable for one year from the date of receipt.

Western Blot (WB)

(All LanesHsp70 family ATPase SSC1 Mouse mAb for the fruit body of yeast at different dilutionLane 1: 1/1000 dilutionLane 2: 1/2000 dilutionLane 3: 1/5000 dilutionLysates/Proteins at 20 ug per lane.Secondary:Goat Anti-Mouse IgG-HRP, 5% skim milk conjugated at 1/100000Predicted Band Size: 70.6 kDaObserved Band Size: 70.6 kDaBlocking/Dilution buffer: 1 x PBS.)

Related Product Information for anti-SSC1 antibody

The protein encoded by SSC1 is the essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in the ATP-dependent manner. Constitutes the ATP-driven core of the motor and binds the precursor preprotein. It is required for the import of the processed fratxin homolog YFH1 into the mitochondrion. The protein acts as a non-catalytic component of endonuclease Scel (Endo>Scel), which cleaves specifically at multiple sites on mitochondrial DNA and produces double-stranded breaks. SSC1 confers broader sequence specificity, greater stability, and higher activity on the catalytic subunit.

Product Categories/Family for anti-SSC1 antibody

Fungi

NCBI and Uniprot Product Information

• NCBI GI #: 6322505
• NCBI GeneID: 853503
• NCBI Accession #: NP_012579.1
• NCBI GenBank Nucleotide #: NM_001181703.1
• Molecular Weight: Predicted MW: 70.6 kDa
• NCBI Official Full Name: Hsp70 family ATPase SSC1
• NCBI Official Symbol: SSC1
• NCBI Official Synonym Symbols: ENS1
• NCBI Protein Information: Hsp70 family ATPase SSC1
• UniProt Protein Name: Heat shock protein SSC1, mitochondrial
• Protein Family: Heat shock protein
• UniProt Gene Name: SSC1
• UniProt Synonym Gene Names: ENS1; Endo.SceI 75 kDa subunit

Uniprot Description

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Constitutes the ATP-driven core of the motor and binds the precursor preprotein. Required for the import of the processed frataxin homolog YFH1 into the mitochondrion.