Human lectin, galactoside-binding, soluble, 13 ELISA Kit | LGALS13 elisa kit

Human placental protein 13, PP13 ELISA Kit

Cat. Num. AAA700610

• Gene Names: LGALS13; PP13; GAL13; PLAC8
• Reactivity: Human
• Synonyms: lectin; galactoside-binding; soluble; 13; Human placental protein 13; PP13 ELISA Kit; GAL13; PLAC8; PP13; beta-galactoside-binding lectin; galectin 13; galectin-13; placental protein 13; LGALS13 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human LGALS13. No significant cross-reactivity or interference between Human LGALS13 and analogues was observed.
• Sequence Length: 139

• Samples: Serum, plasma, urine, tissue homogenates
• Assay Type: Competitive
• Detection Range: 2.5 pg/ml-1000 pg/ml
• Sensitivity: 1 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Biosynthesis/Metabolism
• Protein Biological Process 2: Lipogenesis and lipometabolism
• Protein Biological Process 3: Lipid degradation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LGALS13 elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. Antibody specific for PP13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated PP13. A competitive inhibition reaction is launched between PP13 (Standards or samples) and HRP-conjugated PP13 with the pre-coated antibody specific for PP13. The more amount of PP13 in samples, the less antibody bound by HRP-conjugated PP13. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of PP13 in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 7019497
• NCBI GeneID: 29124
• NCBI Accession #: NP_037400.1
• NCBI GenBank Nucleotide #: NM_013268.2
• UniProt Accession #: Q9UHV8; C5HZ15
• Molecular Weight: 16,119 Da
• NCBI Official Full Name: galactoside-binding soluble lectin 13
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 13
• NCBI Official Symbol: LGALS13
• NCBI Official Synonym Symbols: PP13; GAL13; PLAC8
• NCBI Protein Information: galactoside-binding soluble lectin 13; gal-13; galectin 13; galectin-13; placental protein 13; placental tissue protein 13; beta-galactoside-binding lectin
• UniProt Protein Name: Galactoside-binding soluble lectin 13
• Protein Family: Galactoside-binding soluble lectin
• UniProt Gene Name: LGALS13
• UniProt Synonym Gene Names: PLAC8; Gal-13; PP13; Placental protein 13
• UniProt Entry Name: PP13_HUMAN

NCBI Description

Lysophospholipases are enzymes that act on biological membranes to regulate the multifunctional lysophospholipids. The protein encoded by this gene has lysophospholipase activity. It is composed of two identical subunits which are held together by disulfide bonds. This protein has structural similarity to several members of the beta-galactoside-binding S-type lectin family. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Binds beta-galactoside and lactose. Strong inducer of T-cell apoptosis.2 PublicationsManual assertion based on experiment in:Ref.1