Western Blot (WB)
(Western blot analysis of HSP65: Lane 1: MW marker, Lane 2: GroEL Recombinant E Coli Protein Lane 3: HSP60 Recombinant Human Protein, Lane 4: HSP65 Recombinant Mycobacterium bovis Protein, Lane 5: HeLa (HS) Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: 3T3 Cell Lysate.)
Mouse HSP65 Monoclonal Antibody | anti-Heat shock protein 65 antibody
HSP65 (mycobacterial) monoclonal antibody (3F7)
Reactivity
Human, Mouse, Rat, Bovine, Cryptosporidium, Drosophila, E Coli, Guinea pig, Hamster, Mycobacterium bovis, Mycobacterium leprae
Applications
Western Blot
Purity
Ammonium sulfate precipitation purified.
Synonyms
HSP65; Monoclonal Antibody; HSP65 (mycobacterial) monoclonal antibody (3F7); anti-Heat shock protein 65 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Cryptosporidium, Drosophila, E Coli, Guinea pig, Hamster, Mycobacterium bovis, Mycobacterium leprae
Clonality
Monoclonal
Isotype
IgM
Clone Number
3F7
Specificity
Recognizes human, mouse, rat, bovine, bacteria (E Coli, M. leprae, M. bovis), cryptosporidium, Drosophila, hamster and guinea pig HSP65. Detects a band of ~65kDa by Western blot.
Purity/Purification
Ammonium sulfate precipitation purified.
Form/Format
Liquid. In PBS containing 0.09% sodium azide.
Sequence Length
540
Applicable Applications for anti-Heat shock protein 65 antibody
Western Blot (WB)
Application Notes
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~65kDa by Western blot.
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~65kDa by Western blot.
Immunogen
Recombinant M. bovis HSP65.
Preparation and Storage
Long term storage: 4 degree C
Avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of HSP65: Lane 1: MW marker, Lane 2: GroEL Recombinant E Coli Protein Lane 3: HSP60 Recombinant Human Protein, Lane 4: HSP65 Recombinant Mycobacterium bovis Protein, Lane 5: HeLa (HS) Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: 3T3 Cell Lysate.)
Western Blot (WB)
(Western blot analysis of HSP65: Lane 1: MW marker, Lane 2: GroEL Recombinant E Coli Protein Lane 3: HSP60 Recombinant Human Protein, Lane 4: HSP65 Recombinant Mycobacterium bovis Protein, Lane 5: HeLa (HS) Cell Lysate, Lane 6: PC-12 Cell Lysate, Lane 7: 3T3 Cell Lysate.)
Related Product Information for anti-Heat shock protein 65 antibody
Hsp65 is a member of the Hsp60 family of heat shock proteins isolated from Mycobacterium bovis BCG. Hsp65 from M. bovis is identical to that of M. tuberculosis, and similar to that of M. leprae, two highly pathogenic strains of mycobacterium. Hsp65 is the immunodominant antigen during mycobacterial infection and vaccination, and has been linked to the development of autoimmune adjuvant arthritis in rats.
Product Categories/Family for anti-Heat shock protein 65 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
56,727 Da
NCBI Official Full Name
60 kDa chaperonin 2
NCBI Official Symbol
groEL
NCBI Protein Information
molecular chaperone GroEL
UniProt Protein Name
60 kDa chaperonin 2
UniProt Gene Name
groL2
UniProt Entry Name
CH602_MYCBO
Uniprot Description
Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.
Similar Products
Product Notes
The Heat shock protein 65 grol2 (Catalog #AAA565014) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HSP65 (mycobacterial) monoclonal antibody (3F7) reacts with Human, Mouse, Rat, Bovine, Cryptosporidium, Drosophila, E Coli, Guinea pig, Hamster, Mycobacterium bovis, Mycobacterium leprae and may cross-react with other species as described in the data sheet. AAA Biotech's HSP65 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Detects a band of ~65kDa by Western blot. Researchers should empirically determine the suitability of the Heat shock protein 65 grol2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP65, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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