Rabbit HMG4 Monoclonal Antibody | anti-HMGB3 antibody

HMG4 Rabbit mAb

Cat. Num. AAA4752798

• Gene Names: HMGB3; HMG4; HMG-4; HMG2A; HMG-2a
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity purified
• Synonyms: HMG4; Monoclonal Antibody; HMG4 Rabbit mAb; High mobility group protein B3; HMG-4; HMG2A; HMG-2a; anti-HMGB3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: R01-9C9
• Specificity: Expressed predominantly in placenta. Gene expression databases
• Purity/Purification: Affinity purified
• Form/Format: Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

• Applicable Applications for anti-HMGB3 antibody: Western Blot (WB)
• Application Notes: WB: 1:2000-1:10,000
• Immunogen: A synthetic peptide of human HMG4
• Conjugation: Unconjugated
• Modification: Unmodification
• Subcellular Location: Chromosome, Cytoplasm, Nucleus
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Western blot detection of HMG4 in K562,C6,3T3,Hela cell lysates using HMG4 Rabbit mAb(1:1000 diluted). Predicted band size:23kDa. Observed band size:23kDa.)

Related Product Information for anti-HMGB3 antibody

This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Multifunctional protein with various roles in different cellular compartments. May act in a redox sensitive manner. Associates with chromatin and binds DNA with a preference to non-canonical DNA structures such as single-stranded DNA. Can bent DNA and enhance DNA flexibility by looping thus providing a mechanism to promote activities on various gene promoters. Proposed to be involved in the innate immune response to nucleic acids by acting as a cytoplasmic promiscuous immunogenic DNA/RNA sensor. Negatively regulates B-cell and myeloid cell differentiation. In hematopoietic stem cells may regulate the balance between self-renewal and differentiation. Involved in negative regulation of canonical Wnt signaling.

NCBI and Uniprot Product Information

• NCBI GI #: 85701353
• NCBI GeneID: 3149
• UniProt Accession #: O15347; O95556; Q6NS40
• Molecular Weight: 200
• NCBI Official Full Name: High mobility group protein B3
• NCBI Official Synonym Full Names: high mobility group box 3
• NCBI Official Symbol: HMGB3
• NCBI Official Synonym Symbols: HMG4; HMG-4; HMG2A; HMG-2a
• NCBI Protein Information: high mobility group protein B3; high mobility group protein 2a; high-mobility group (nonhistone chromosomal) protein 4
• UniProt Protein Name: High mobility group protein B3
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGB3
• UniProt Synonym Gene Names: HMG2A; HMG4; HMG-2a; HMG-4
• UniProt Entry Name: HMGB3_HUMAN

NCBI Description

This gene encodes a member of a family of proteins containing one or more high mobility group DNA-binding motifs. The encoded protein plays an important role in maintaining stem cell populations, and may be aberrantly expressed in tumor cells. A mutation in this gene was associated with microphthalmia, syndromic 13. There are numerous pseudogenes of this gene on multiple chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

HMGB3: Binds preferentially single-stranded DNA and unwinds double stranded DNA. Belongs to the HMGB family.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: chromosome; nucleus

Molecular Function: double-stranded DNA binding; chromatin binding; DNA bending activity

Biological Process: chromatin remodeling; negative regulation of B cell differentiation; negative regulation of myeloid cell differentiation; regulation of transcription, DNA-dependent; multicellular organismal development; DNA recombination

Disease: Microphthalmia, Syndromic 13