Mouse anti-Human HMG1 / HMGB1 Monoclonal Antibody | anti-HMG1 antibody

Mouse Monoclonal [clone 2F6] (IgG2a,k) to Human HMG1 / HMGB1

Cat. Num. AAA246467

• Gene Names: HMGB1; HMG1; HMG3; SBP-1
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: HMG1 / HMGB1; Monoclonal Antibody; Mouse Monoclonal [clone 2F6] (IgG2a; k) to Human HMG1 / HMGB1; Anti-HMG1 / HMGB1 Antibody (clone 2F6) IHC-plus; HMGB1; Amphoterin; High mobility group protein B1; High mobility group box 1; High mobility group protein 1; HMG1; HMG3; SBP-1; High-mobility group box 1; HMG-1; Human HMG1; anti-HMG1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2F6
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 215

• Applicable Applications for anti-HMG1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB; Usage: Western Blot (Cell lysate) - positive control Hela NE. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 ug/ml) - positive control HeLa cells.
• Target Species: Human
• Immunogen Description: HMGB1 (NP_002119, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: HMG1 / HMGB1 antibody was raised against hMGB1 (NP_002119, 1 a.a. ~ 91 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-HMGB1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-HMGB1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-HMGB1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(Western blot of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody, clone 2F6.)

Western Blot (WB)

(HMGB1 monoclonal antibody, clone 2F6 Western blot of HMGB1 expression in HeLa NE.)

ELISA (EIA)

(Detection limit for recombinant GST tagged HMGB1 is approximately 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 4504425
• NCBI GeneID: 3146
• NCBI Accession #: NP_002119.1
• NCBI GenBank Nucleotide #: NM_002128.4
• UniProt Accession #: P09429; Q14321; Q5T7C3; Q6IBE1; A5D8W9
• Molecular Weight: 24,894 Da
• NCBI Official Full Name: high mobility group protein B1
• NCBI Official Synonym Full Names: high mobility group box 1
• NCBI Official Symbol: HMGB1
• NCBI Official Synonym Symbols: HMG1; HMG3; SBP-1
• NCBI Protein Information: high mobility group protein B1; HMG-1; Amphoterin; high-mobility group box 1; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1
• UniProt Protein Name: High mobility group protein B1
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGB1
• UniProt Synonym Gene Names: HMG1; HMG-1
• UniProt Entry Name: HMGB1_HUMAN

Uniprot Description

HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family.

Protein type: Nuclear receptor co-regulator; DNA repair, damage

Chromosomal Location of Human Ortholog: 13q12

Cellular Component: nucleoplasm; extracellular space; cell surface; transcriptional repressor complex; extracellular region; condensed chromosome; nucleus

Molecular Function: protein binding; RAGE receptor binding; double-stranded DNA binding; cytokine activity; damaged DNA binding; chromatin binding; transcription factor binding; transcription factor activity; DNA bending activity; single-stranded DNA binding; chemoattractant activity

Biological Process: negative regulation of transcriptional preinitiation complex assembly; V(D)J recombination; DNA topological change; positive regulation of apoptosis; apoptosis; positive regulation of caspase activity; base-excision repair, DNA ligation; negative regulation of transcription from RNA polymerase II promoter; DNA recombination; regulation of transcription from RNA polymerase II promoter; chromatin remodeling; myeloid dendritic cell activation; inflammatory response to antigenic stimulus; positive chemotaxis; dendritic cell chemotaxis; innate immune response; DNA ligation during DNA repair; positive regulation of transcription from RNA polymerase II promoter; DNA fragmentation during apoptosis; neurite development; cell structure disassembly during apoptosis; positive regulation of DNA binding