Mouse GATA4 Monoclonal Antibody | anti-GATA4 antibody

GATA4 antibody

Cat. Num. AAA830379

• Gene Names: GATA4; TOF; ASD2; VSD1; TACHD
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: GATA4 antibody was purified by affinity chromatography.
• Synonyms: GATA4; Monoclonal Antibody; GATA4 antibody; Monoclonal GATA4; Anti-GATA4; GATA 4; GATA-4; anti-GATA4 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3
• Clone Number: 9F9
• Purity/Purification: GATA4 antibody was purified by affinity chromatography.
• Form/Format: Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 500 ug-1 mg/ml (varies by lot)
• Sequence Length: 442

• Applicable Applications for anti-GATA4 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50; WB: 1:200-500; IF: 1:100
• Biological Significance: GATA4 is a member of the GATA family of zinc-finger transcription factors. Members of this family recognizethe GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. Mutations in this gene have been associated with cardiacseptal defects.
• Cross-Reactivity: Human
• Immunogen: GATA4 antibody was raised in mouse using a human recombinant protein fragment corresponding to amino acids 296-442 of human GATA4 (NP_002043) produced in E Coli as the immunogen.
• Preparation and Storage: Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant GATA4 protein (Right) or empty vector (Left) detected with GATA4 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of GATA4 protein in paraffin embedded Human endometrium tissue using GATA4 antibody)

Related Product Information for anti-GATA4 antibody

Mouse monoclonal GATA4 antibody

Product Categories/Family for anti-GATA4 antibody

DNA & RNA

NCBI and Uniprot Product Information

• NCBI GI #: 33188461
• NCBI GeneID: 2626
• NCBI Accession #: NP_002043
• NCBI GenBank Nucleotide #: NM_002052.3
• UniProt Accession #: P43694; Q3MJ45; Q5IFM8; B7ZKX0; B7ZKZ4
• Molecular Weight: 44,665 Da
• NCBI Official Full Name: transcription factor GATA-4 isoform 2
• NCBI Official Synonym Full Names: GATA binding protein 4
• NCBI Official Symbol: GATA4
• NCBI Official Synonym Symbols: TOF; ASD2; VSD1; TACHD
• NCBI Protein Information: transcription factor GATA-4
• UniProt Protein Name: Transcription factor GATA-4
• Protein Family: GATA transcription factor
• UniProt Gene Name: GATA4
• UniProt Entry Name: GATA4_HUMAN

NCBI Description

This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

GATA4: a conserved and ubiquitous member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. May regulate a set of cardiac-specific genes and play a crucial role in cardiogenesis. Defects are a cause of atrial septal defect 2 (ASD2). ASD2 is an autosomal dominant condition with atrial septal defect and other congenital heart disease but no conduction defects or noncardiac abnormalities.

Protein type: Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 8p23.1-p22

Cellular Component: nucleoplasm; nuclear chromatin; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; NFAT protein binding; DNA binding; transcription activator binding; zinc ion binding; sequence-specific DNA binding; transcription coactivator activity; chromatin binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; cardiac muscle hypertrophy; negative regulation of autophagy; embryonic heart tube anterior/posterior pattern formation; positive regulation of transcription, DNA-dependent; Sertoli cell differentiation; Wnt receptor signaling pathway through beta-catenin; BMP signaling pathway; regulation of transcription, DNA-dependent; cell-cell signaling; embryonic digestive tract morphogenesis; embryonic foregut morphogenesis; heart looping; positive regulation of BMP signaling pathway; positive regulation of cardiac muscle cell proliferation; response to drug; response to retinoic acid; in utero embryonic development; male gonad development; gastrulation with mouth forming second; positive regulation of angiogenesis; endoderm formation; response to estrogen stimulus; response to mechanical stimulus; positive regulation of cardioblast differentiation; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; endoderm development

Disease: Ventricular Septal Defect 1; Atrial Septal Defect 2; Tetralogy Of Fallot; Atrioventricular Septal Defect 4; Testicular Anomalies With Or Without Congenital Heart Disease