Mouse anti-Human HLA-A2 Monoclonal Antibody | anti-HLA-A2 antibody

Anti-HLA-A2 Antibody (clone BB7.2, Low Endotoxin)

Cat. Num. AAA2401007

• Gene Names: HLA-A; HLAA
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Functional Assay, Immunohistochemistry, Immunoprecipitation
• Purity: Protein G purified
• Synonyms: HLA-A2; Monoclonal Antibody; Anti-HLA-A2 Antibody (clone BB7.2; Low Endotoxin); Mouse Monoclonal [clone BB7.2] (IgG2b) to Human HLA-A2; anti-HLA-A2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: BB7.2
• Specificity: The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. The HLA A gene is part of the human HLA complex on chromosome 6 and there are a large number of variant alleles of this gene. The antibody recognizes the human HLA A2 histocompatability antigen. The epitope recognized by this has been studied extensively and would appear to include the carboxy-terminus of the alpha-2 helix and a turn on one of the underlying beta strands. The antibody may be used for the flow cytometric detection of HLA A2 expression and has also been used for immunoaffinity purification of HLA A2 molecules. Functionally this is reported to inhibit MHC restricted cellular cytotoxicity.
• Purity/Purification: Protein G purified
• Sequence Length: 365

• Applicable Applications for anti-HLA-A2 antibody: Flow Cytometry (FC/FACS), Functional Assay, Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP)
• Application Notes: FC/FACS: 10 ug/ml; Func: 5-10 ug/ml; IHC-P: 10 ug/ml
• Immunogen: Papain solubilized HLA-A2.
• Conjugation: Low Endotoxin Unconjugated
• Preparation and Storage: Store at -20 degree C.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-HLA-A2 antibody IHC staining of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. This image was taken for the unmodified form of this product. Other forms have not been tested.)

Flow Cytometry (FC/FACS)

(Flow cytometry of human peripheral blood lymphocytes with Mouse anti-Human HLA-A2: endotoxin low This image was taken for the unmodified form of this product. Other forms have not been tested.)

NCBI and Uniprot Product Information

• NCBI GI #: 33187148
• NCBI GeneID: 3105
• Molecular Weight: 40,909 Da
• NCBI Official Full Name: HLA-A2
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, A
• NCBI Official Symbol: HLA-A
• NCBI Official Synonym Symbols: HLAA
• NCBI Protein Information: HLA class I histocompatibility antigen, A-1 alpha chain; LOW QUALITY PROTEIN: HLA class I histocompatibility antigen, A-1 alpha chain
• UniProt Protein Name: HLA class I histocompatibility antigen, A-68 alpha chain
• UniProt Gene Name: HLA-A
• UniProt Synonym Gene Names: HLAA

NCBI Description

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the presentation of foreign antigens to the immune system.