Mouse HIV-1 gp120, Strain IIIB Monoclonal Antibody
Mouse anti-HIV-1 gp120, Strain IIIB (Human Immunodeficiency Virus Type 1) (FITC)
Applications
Dot Blot, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
>95% Purified by gel exclusion chromatography.
Synonyms
HIV-1 gp120; Strain IIIB; Monoclonal Antibody; Mouse anti-HIV-1 gp120; Strain IIIB (Human Immunodeficiency Virus Type 1) (FITC); anti-HIV-1 gp120; Strain IIIB antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
5i20
Specificity
Recognizes native and recombinant HIV-1 gp120. Stains cell surfaces in direct IF assays.
Specific for the V3 loop of HIV-1 IIIB gp120/gp160 and shows 10-50% cross-reactivity with other HIV-1 strains.
Specific for the V3 loop of HIV-1 IIIB gp120/gp160 and shows 10-50% cross-reactivity with other HIV-1 strains.
Purity/Purification
>95% Purified by gel exclusion chromatography.
Form/Format
Supplied as a liquid in 10mM Tris, pH 8.0. No stabilizers or preservatives added. Labeled with fluorescein isothiocyanate (FITC).
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-HIV-1 gp120, Strain IIIB antibody
Dot Blot (DB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) and Direct Immunofluorescence (IF)
Application Notes
Direct Immunofluorescence: 1:50
Dot Blot: 1:200
Other applications not tested. Optimal dilutions to be determined by the researcher.
Dot Blot: 1:200
Other applications not tested. Optimal dilutions to be determined by the researcher.
Immunogen
HIV-1 gp120
Grade
Highly Purified
F/P Ratio
3-4
Preparation and Storage
Store product at 4°C if to used immediately within 2 weeks. For long term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-HIV-1 gp120, Strain IIIB antibody
HIV is a member of the genus Lentivirus, part of the family of Retroviridae, which use reverse transcriptase to convert the viral RNA genome into double stranded DNA. This is then integrated into the cell's DNA, which is followed by latency or active HIV replication. HIV primarily infects CD4+ T lymphocytes, resulting in their dramatic decline. When CD4+ T cell numbers decline below a critical level, cell-mediated immunity is lost, and the body becomes progressively more susceptible to opportunistic infections. If untreated, this can lead to AIDS (Acquired Immunodeficiency Syndrome). Human immunodeficiency virus type 1 (HIV-1) entry into target cells is directed by the envelope (Env) glycoproteins, which are present on the surface of HIV-1 virion or infected cells in the form of trimers consisting of gp120/gp41 complexes. The surface subunit, gp120, initiates the entry process by interacting sequentially with the CD4 receptor and a co-receptor CCR5 or CXCR4, thereby inducing a conformational change that allows the transmembrane (TM) gp41 subunit to mediate fusion between viral and target cell membranes. Cleavage of Env into its gp120 and gp41 components is necessary for activation of its fusogenic activity.
Product Categories/Family for anti-HIV-1 gp120, Strain IIIB antibody
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Product Notes
The HIV-1 gp120, Strain IIIB (Catalog #AAA632531) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HIV-1 gp120, Strain IIIB can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) and Direct Immunofluorescence (IF). Direct Immunofluorescence: 1:50 Dot Blot: 1:200 Other applications not tested. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the HIV-1 gp120, Strain IIIB for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIV-1 gp120, Strain IIIB, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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