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Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) Raw264.7, 3) Mouse brain, 4) Rat brain using Histone H3 Monoclonal Antibody at dilution of 1:5000.)
Methyl-Histone H3 Antibody Kit
Methyl-Histone H3 Antibody Sampler Kit
Applications
Western Blot
Synonyms
Methyl-Histone H3; Methyl-Histone H3 Antibody Sampler Kit; Methyl-Histone H3 antibody kit
Applicable Applications for Methyl-Histone H3 antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) Raw264.7, 3) Mouse brain, 4) Rat brain using Histone H3 Monoclonal Antibody at dilution of 1:5000.)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) Raw264.7, 3) Mouse brain, 4) Rat brain using Histone H3 Monoclonal Antibody at dilution of 1:5000.)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) 3T3, 3) Raw264.7 cells using Histone H3 (Di Methyl Lys4) Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) 3T3, 3) Raw264.7 cells using Histone H3 (Di Methyl Lys4) Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) Rat heart, 3) Raw264.7 using Histone H3 (Di Methyl Lys9) Monoclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) Rat heart, 3) Raw264.7 using Histone H3 (Di Methyl Lys9) Monoclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) Raw264.7, 3) Rat brain using Histone H3 (Di Methyl Lys36) Monoclonal Antibody at dilution of 1:3000.)
Western Blot (WB)
(Western Blot analysis of 1) Hela, 2) Raw264.7, 3) Rat brain using Histone H3 (Di Methyl Lys36) Monoclonal Antibody at dilution of 1:3000.)
Western Blot (WB)
(Western Blot analysis of Hela cells using Histone H3 (Di Methyl Lys27) Monoclonal Antibody at dilution of)
Western Blot (WB)
(Western Blot analysis of Hela cells using Histone H3 (Di Methyl Lys27) Monoclonal Antibody at dilution of)
Western Blot (WB)
(2) 1:3000.)
Western Blot (WB)
(2) 1:3000.)
Western Blot (WB)
(Western Blot analysis of Hela cells using Histone H3 (Di Methyl Lys79) Monoclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of Hela cells using Histone H3 (Di Methyl Lys79) Monoclonal Antibody at dilution of 1:2000.)
Related Product Information for Methyl-Histone H3 antibody kit
Background: The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post- translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development. Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker.
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Product Notes
The Methyl-Histone H3 (Catalog #AAA2550118) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Methyl-Histone H3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the Methyl-Histone H3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Methyl-Histone H3, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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