Western Blot (WB)
(Western blot detection of Hexokinase 1 in MCF7,Jurkat,CHO-K1,C2C12,C6,3T3 and Hela cell lysates using Hexokinase 1 mouse mAb (1:1000 diluted). Predicted band size:102KDa. Observed band size:102KDa.)
Mouse anti-Human, Mouse Hexokinase 1 Monoclonal Antibody | anti-HK1 antibody
Hexokinase 1 Antibody
Gene Names
HK1; HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
Reactivity
Human, Mouse
Applications
Western Blot
Synonyms
Hexokinase 1; Monoclonal Antibody; Hexokinase 1 Antibody; Purified Mouse Monoclonal Antibody (Mab); BB404130; Brain form hexokinase; dea; EC 2.7.1.1; Glycolytic enzyme; HEXOKIN; Hexokinase PI; Hexokinase type I; Hexokinase; tumor isozyme; Hexokinase-1; Hexokinase-A; HK I; HK1; HK1 tb; Hk1-s; HK1-ta; HK1-tb; HK1-tc; HKD; HKI; HMSNR; HXK1; HXK1_HUMAN; mHk1-s; anti-HK1 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Form/Format
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Sequence Length
917
Applicable Applications for anti-HK1 antibody
Western Blot (WB)
Application Notes
WB ~~ 1:1000
Antigen Source
Human
Antigen Type
Recombinant Protein
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Western blot detection of Hexokinase 1 in MCF7,Jurkat,CHO-K1,C2C12,C6,3T3 and Hela cell lysates using Hexokinase 1 mouse mAb (1:1000 diluted). Predicted band size:102KDa. Observed band size:102KDa.)
Western Blot (WB)
(Western blot detection of Hexokinase 1 in MCF7,Jurkat,CHO-K1,C2C12,C6,3T3 and Hela cell lysates using Hexokinase 1 mouse mAb (1:1000 diluted). Predicted band size:102KDa. Observed band size:102KDa.)
Product Categories/Family for anti-HK1 antibody
References
Nishi S.,et al.Biochem. Biophys. Res. Commun. 157:937-943(1988).
Ruzzo A.,et al.Biochem. J. 331:607-613(1998).
Deloukas P.,et al.Nature 429:375-381(2004).
Andreoni F.,et al.Biochim. Biophys. Acta 1493:19-26(2000).
Murakami K.,et al.Blood 90:272-272(1998).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
102486
NCBI Official Full Name
hexokinase-1 isoform HKI
NCBI Official Synonym Full Names
hexokinase 1
NCBI Official Symbol
HK1
NCBI Official Synonym Symbols
HKD; HKI; HXK1; HMSNR; HK1-ta; HK1-tb; HK1-tc
NCBI Protein Information
hexokinase-1
UniProt Protein Name
Hexokinase-1
Protein Family
UniProt Gene Name
HK1
UniProt Synonym Gene Names
HK I
UniProt Entry Name
HXK1_HUMAN
NCBI Description
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]
Research Articles on HK1
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Product Notes
The HK1 hk1 (Catalog #AAA9215425) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Hexokinase 1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Hexokinase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB ~~ 1:1000. Researchers should empirically determine the suitability of the HK1 hk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hexokinase 1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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