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Western Blot (WB) (The cell lysates of MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HEXA antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HEXA antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HEXA Transfected 293T cell lysate)

Mouse anti-Human HEXA Monoclonal Antibody | anti-HEXA antibody

HEXA antibody

Gene Names
HEXA; TSD
Reactivity
Human
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
By protein-A affinity chromatography
Synonyms
HEXA; Monoclonal Antibody; HEXA antibody; beta-hexosaminidase subunit alpha; TSD; hexosaminidase A; anti-HEXA antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, l
Clone Number
AT20F1
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
529
Applicable Applications for anti-HEXA antibody
ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Recommended starting dilution is 1:3000.
Antigen Species
Human
Immunogen
Recombinant human HEXA (89-529aa) purified from E. coli
Clone Note
Anti-human HEXA mAb, clone AT20F1, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human HEXA protein.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The cell lysates of MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HEXA antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HEXA antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HEXA Transfected 293T cell lysate)

Western Blot (WB) (The cell lysates of MCF7 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HEXA antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HEXA antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: HEXA Transfected 293T cell lysate)
Related Product Information for anti-HEXA antibody
HEXA (Hexosaminidase A), also designated beta-Hexosaminidase A, is responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. A mutation in the a subunit of hexosaminidase is the cause of Tay-Sachs disease (TSD), also known as GM2-gangliosidosis type I. TSD is a fatal autosomal recessive lysosomal storage disease of the central nervous system (CNS) caused by insufficient activity of the HEXA enzyme that results in a failure to process GM2 gangliosides. The accumulation of GM2 ganglioside in the absence of HEXA activity causes progressive destruction of the CNS.
Product Categories/Family for anti-HEXA antibody
References
Cabezas. J.A. (1989) Biochem J 261 (3): 1059-1060; Lemieux. M.J., et al. (2006) J Mol Biol 359 (4): 913-929; Boles. D.J. and Proia. R.L. (1995) Am J Hum Genet 56 (3): 716-724

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,326 Da
NCBI Official Full Name
hexosaminidase A preproprotein
NCBI Official Synonym Full Names
hexosaminidase subunit alpha
NCBI Official Symbol
HEXA
NCBI Official Synonym Symbols
TSD
NCBI Protein Information
beta-hexosaminidase subunit alpha
UniProt Protein Name
Beta-hexosaminidase subunit alpha
Protein Family
UniProt Gene Name
HEXA
UniProt Synonym Gene Names
Hexosaminidase subunit A

NCBI Description

This gene encodes a member of the glycosyl hydrolase 20 family of proteins. The encoded preproprotein is proteolytically processed to generate the alpha subunit of the lysosomal enzyme beta-hexosaminidase. This enzyme, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. Mutations in this gene lead to an accumulation of GM2 ganglioside in neurons, the underlying cause of neurodegenerative disorders termed the GM2 gangliosidoses, including Tay-Sachs disease (GM2-gangliosidosis type I). Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. The form B is active against certain oligosaccharides. The form S has no measurable activity.

Research Articles on HEXA

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Product Notes

The HEXA hexa (Catalog #AAA200278) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HEXA antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HEXA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Recommended starting dilution is 1:3000. Researchers should empirically determine the suitability of the HEXA hexa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HEXA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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