Rabbit anti-Human VLDLR Polyclonal Antibody | anti-VLDLR antibody

VLDLR, ID (VLDLR, Very low-density lipoprotein receptor) (FITC)

Cat. Num. AAA6362645

• Gene Names: VLDLR; CARMQ1; CHRMQ1; VLDLRCH
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: VLDLR; Polyclonal Antibody; ID (VLDLR; Very low-density lipoprotein receptor) (FITC); Very low-density lipoprotein receptor; anti-VLDLR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VLDLR.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-VLDLR antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: IHC-P: Formalin-fixed, paraffin-embedded. Requires HIER using sodium citrate buffer pH6.0 prior to staining.; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 484-510 in the central region of human VLDLR, UniProt Accession #P98155. Species sequence homology: rabbit
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell line lysates (35ug/lane) usingMBS641833. This demonstrates that MBS641833 detected the VLDLR protein (arrow).)

Western Blot (WB)

(Western Blot analysis in human heart lysate (20ug) using MBS641833 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000 dilution. Predicted band size: 96kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed usingMBS641833; antigen retrieval was by heat mediation with a citrate buffer (pH 6) follow by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS641833 for immunohistochemistry.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed paraffin embedded human heart tissue sections usingMBS641833. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5hrs at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with MBS641833 (1:25) for 1 hour at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of 293 cells using MBS641833 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-VLDLR antibody

Antibodies; Lipoproteins

References

1. Sakai, K., et al. Brain Res. 1276, 11-21 (2009). 2. Francis, P.J., et al. J. Med. Genet. 46(5):300-307(2009). 3. Ananyeva, N.M., et al. Blood Coagul. Fibrinolysis 19(6):543-555(2008). 4. Turkmen, S., et al. Eur. J. Hum. Genet. 16(9):1070-1074(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 65301167
• NCBI GeneID: 7436
• NCBI Accession #: NP_003374.3
• NCBI GenBank Nucleotide #: NM_003383.3
• UniProt Accession #: P98155; Q5VVF6; B2RMZ7; D3DRH6
• Molecular Weight: 96,098 Da
• NCBI Official Full Name: very low-density lipoprotein receptor isoform a
• NCBI Official Synonym Full Names: very low density lipoprotein receptor
• NCBI Official Symbol: VLDLR
• NCBI Official Synonym Symbols: CARMQ1; CHRMQ1; VLDLRCH
• NCBI Protein Information: very low-density lipoprotein receptor; VLDL-R; VLDL receptor
• UniProt Protein Name: Very low-density lipoprotein receptor
• Protein Family: Very low-density lipoprotein receptor
• UniProt Gene Name: VLDLR
• UniProt Synonym Gene Names: VLDL receptor; VLDL-R
• UniProt Entry Name: VLDLR_HUMAN

NCBI Description

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. This gene encodes a lipoprotein receptor that is a member of the LDLR family and plays important roles in VLDL-triglyceride metabolism and the reelin signaling pathway. Mutations in this gene cause VLDLR-associated cerebellar hypoplasia. Alternative splicing generates multiple transcript variants encoding distinct isoforms for this gene. [provided by RefSeq, Aug 2009]

Uniprot Description

VLDLR: Binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation. Defects in VLDLR are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 1 (CMARQ1); also known as dysequilibrium syndrome (DES) or non- progressive cerebellar disorder with mental retardation. CMARQ1 is a congenital, non-progressive cerebellar ataxia associated with disturbed equilibrium, delayed ambulation, mental retardation and cerebellar hypoplasia. Additional features include short stature, strabismus, pes planus and, rarely, seizures. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 9p24

Cellular Component: membrane; plasma membrane; integral to membrane; coated pit; receptor complex

Molecular Function: very-low-density lipoprotein receptor activity; low-density lipoprotein receptor activity; very-low-density lipoprotein binding; protein binding; apolipoprotein binding; calcium ion binding; glycoprotein binding; calcium-dependent protein binding

Biological Process: nervous system development; receptor-mediated endocytosis; cholesterol metabolic process; positive regulation of protein kinase activity; ventral spinal cord development; negative regulation of transcription from RNA polymerase II promoter; signal transduction; lipid transport; memory

Disease: Cerebellar Ataxia, Mental Retardation, And Dysequilibrium Syndrome 1