Testing Data
(Figure 1: Immunohistochemistry on frozen section of Human kidney showing strong reactivity in the extracellular matrix and basement membrane.
)
Rat Heparan Sulphate Proteoglycan Monoclonal Antibody | anti-GPC3 antibody
Rat anti Heparan Sulphate Proteoglycan
Testing Data
(Figure 1: Immunohistochemistry on frozen section of Human kidney showing strong reactivity in the extracellular matrix and basement membrane. )
Testing Data
(Figure 1: Immunohistochemistry on frozen section of Human kidney showing strong reactivity in the extracellular matrix and basement membrane.
)
NCBI and Uniprot Product Information
NCBI Description
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
Uniprot Description
GPC3: Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition. Defects in GPC3 are the cause of Simpson-Golabi-Behmel syndrome type 1 (SGBS1); also known as Simpson dysmorphia syndrome (SDYS). SGBS is a condition characterized by pre- and postnatal overgrowth (gigantism) with visceral and skeletal anomalies. Belongs to the glypican family.
Protein type: Membrane protein, GPI anchor; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: Xq26.1
Cellular Component: lysosomal lumen; proteinaceous extracellular matrix; extracellular space; anchored to plasma membrane; integral to plasma membrane; Golgi lumen; plasma membrane
Molecular Function: heparan sulfate proteoglycan binding; protein binding
Biological Process: phototransduction, visible light; anatomical structure morphogenesis; glycosaminoglycan metabolic process; negative regulation of peptidase activity; pathogenesis; positive regulation of endocytosis; osteoclast differentiation; embryonic hindlimb morphogenesis; body morphogenesis; bone mineralization; chondroitin sulfate metabolic process; positive regulation of glucose import; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; ureteric bud branching; negative regulation of smoothened signaling pathway; carbohydrate metabolic process; positive regulation of protein catabolic process; positive regulation of smoothened signaling pathway; retinoid metabolic process; positive regulation of BMP signaling pathway; lung development; negative regulation of epithelial cell proliferation; anterior/posterior axis specification; negative regulation of growth
Disease: Simpson-golabi-behmel Syndrome, Type 1; Wilms Tumor 1
Research Articles on GPC3
2. Data show that mutated GPC3 lacking the GPI anchoring domain (sGPC3) significantly inhibited the in vivo growth, blocked Wnt signaling and Erk1/2 and Akt phosphorylation in tumors.
3. Our data supports the potentially significant diagnostic utility of GPC-3 in fine-needle aspiration biopsies in differentiating primary malignant from benign and preneoplastic liver lesions, and metastatic carcinomas.
4. Report the expression profile of glypican-3 and its relation to macrophage population in human hepatocellular carcinoma.
5. GPC3 expression is correlated with poor prognosis in hepatocellular carcinoma.
6. overexpression of glypican-3 may be related to the development and aggressive behavior of ovarian clear cell adenocarcinoma.
7. Detection of glypican-3-specific CTLs in chronic hepatitis and liver cirrhosis.
8. A reliable marker for the detection of hepatocellular carcinoma in liver biopsies.
9. A patient with the Simpson-Golabi-Behmel syndrome displays a loss-of-function point mutation in GPC3 that inhibits the attachment of this proteoglycan to the cell surface.
10. GPC3 is frequently expressed in noncutaneous small cell neuroendocrine carcinoma of various origins, in particular in Merkel cell carcinoma, which, in combination with CK20
11. The effector cells stimulated with DCs that were transfected with pEF-hGPC3 plasmid could effectively lyse GPC3 expressing HepG2 cells.
12. GPC3 expression in HCCs did not correlate with the size, differentiation, or stage of the tumors; the presence or absence of cirrhotic background; or the underlying etiologies
13. Report expression pattern of glypican-3 (GPC3) during human embryonic and fetal development.
14. Glypican-3 expression is a potential candidate marker for early detection of lung squamous cell carcinoma.
15. Glypican-3 appears to be a reliable markers for ovarian germ-cell tumors.
16. GPC3 confers oncogenecity through the interaction between IGF-II and its receptor, and the subsequent activation of the IGF-signaling pathway.
17. GPC3 is expressed at an early stage, suggesting that GPC3 expression in thyroid cancer is an early event in developing papillary carcinoma.
18. staining for glypican-3 is a highly sensitive and specific method capable of distinguishing HCC from other benign and malignant hepatic lesions
19. GPC3 seems to be a promising diagnostic marker for differentiating yolk sac tumors from ovarian clear cell carcinomas.
20. GPC3 may play a role in the tumorigenesis of hepatoblastoma.
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Product Notes
The GPC3 gpc3 (Catalog #AAA570091) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat anti Heparan Sulphate Proteoglycan reacts with Bovine, Human, Mouse, Rat, Cod and Wolffish. and may cross-react with other species as described in the data sheet. AAA Biotech's Heparan Sulphate Proteoglycan can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Immunoprecipitation (IP), Western Blot (WB). A7L6 is useful for immunoprecipitation, immunoblotting, immunocytochemistry and immuno-histochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titRation; recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the GPC3 gpc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heparan Sulphate Proteoglycan, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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