Mouse Heat Shock Protein 90, beta Monoclonal Antibody
Heat Shock Protein 90, beta (HSP90b)
Reactivity
Bovine, Canine, Goat, Guinea Pig, Hamster, Human, Mouse, Porcine, Rabbit, Rat
Applications
Western Blot
Purity
Purified by ammonium sulfate precipitation.
Synonyms
Heat Shock Protein 90; beta; Monoclonal Antibody; beta (HSP90b); Anti -Heat Shock Protein 90; anti-Heat Shock Protein 90; beta antibody
Host
Mouse
Reactivity
Bovine, Canine, Goat, Guinea Pig, Hamster, Human, Mouse, Porcine, Rabbit, Rat
Clonality
Monoclonal
Isotype
IgM
Clone Number
4E133
Specificity
This purified antibody identifies purified human Hsp90beta and will detect a 90kD protein corresponding to the apparent molecular mass of Hsp90beta on SDS-PAGE immunoblots in samples from human, mouse, rat, hamster, guinea pig, bovine, canine, goat, porcine and rabbit. This antibody is specific for Hsp90beta and does not detect Hsp90alpha.
Purity/Purification
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, pH 6.9 containing 0.1% sodium azide.
Applicable Applications for anti-Heat Shock Protein 90, beta antibody
Western Blot (WB)
Application Notes
Western Blot: (Colorimetric): 0.5ug/ml, Optimal dilutions to be determined by researcher.
Immunogen
Purified recombinant human Hsp90beta
Positive Control
HeLa Cell Lysate
Preparation and Storage
-20 degree C
Related Product Information for anti-Heat Shock Protein 90, beta antibody
The 90kD molecular chaperone family comprises several proteins including the 90 kD heat shock protein, Hsp90 and the 94 kD glucose-regulated protein, grp94 which are major molecular chaperones of the cytosol and of the endoplasmic reticulum. In mammalian cells there are at least two Hsp90 isoforms, Hsp90 alpha and Hsp90beta which are encoded by separate genes. The amino acid sequence of human and yeast Hsp90alpha is 85% and 90% homologous to that of Hsp90beta respectively (2). All known members of the Hsp90 protein family are highly conserved, especially in the N-terminal and C-terminal regions which have been shown to contain independent chaperone sites with different substrate specificity (3,4). These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cellís powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by non-physiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steroid receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90 (5). It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways. Recent studies indicate that when Drosophila Hsp90 buffering is compromised by e.g., temperature, cryptic variants are expressed and selection can lead to the continued expression of these traits, even if Hsp90 function is restored (6).
Product Categories/Family for anti-Heat Shock Protein 90, beta antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
heat shock protein 90
Similar Products
Product Notes
The Heat Shock Protein 90, beta (Catalog #AAA609565) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Heat Shock Protein 90, beta (HSP90b) reacts with Bovine, Canine, Goat, Guinea Pig, Hamster, Human, Mouse, Porcine, Rabbit, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Heat Shock Protein 90, beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: (Colorimetric): 0.5ug/ml, Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the Heat Shock Protein 90, beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Heat Shock Protein 90, beta, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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