Rabbit anti-Human HCLS1 Polyclonal Antibody | anti-HCLS1 antibody

HCLS1 Rabbit Polyclonal

Cat. Num. AAA7600019

• Gene Names: HCLS1; HS1; p75; CTTNL; lckBP1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: HCLS1; Polyclonal Antibody; HCLS1 Rabbit Polyclonal; anti-HCLS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 449

• Applicable Applications for anti-HCLS1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Hematopoietic cell-specific Lyn substrate 1
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse kidney using MBS7600019 (HCLS1 Antibody) at dilution of 1:50)

Western Blot (WB)

(Jurkat cells were subjected to SDS PAGE followed by western blot with MBS7600019 (HCLS1 Antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 635574580
• NCBI GeneID: 3059
• NCBI Accession #: NP_001278970.1
• NCBI GenBank Nucleotide #: NM_001292041.1
• Molecular Weight: 24,008 Da
• NCBI Official Full Name: hematopoietic lineage cell-specific protein isoform 2
• NCBI Official Synonym Full Names: hematopoietic cell-specific Lyn substrate 1
• NCBI Official Symbol: HCLS1
• NCBI Official Synonym Symbols: HS1; p75; CTTNL; lckBP1
• NCBI Protein Information: hematopoietic lineage cell-specific protein
• UniProt Protein Name: Hematopoietic lineage cell-specific protein
• Protein Family: HCLS1-binding protein
• UniProt Gene Name: HCLS1
• UniProt Synonym Gene Names: HS1
• UniProt Entry Name: HCLS1_HUMAN

Uniprot Description

HS1: Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression. Associates with the SH2 and SH3 domains of LCK. Binding to he LCK SH3 domain occurs constitutively, while binding to the LCK SH2 domain occurs only upon TCR stimulation. A similar binding pattern was observed with LYN, but not with FYN in which the FYN SH2 region associates upon TCR stimulation but the FYN SH3 region does not associate regardless of TCR stimulation. Directly associates with HAX1, through binding to its C-terminal region. Interacts with HS1BP3. Interacts with FES/FPS. Interacts (via SH2 domain) with FGR. Forms a multiprotein complex with LYN and ANKRD54. Expressed only in tissues and cells of hematopoietic origin.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q13

Cellular Component: cytoplasm; membrane; mitochondrion; nucleus; transcription factor complex

Molecular Function: actin binding; protein binding; protein complex binding; protein kinase binding; SH3 domain binding

Biological Process: actin filament polymerization; erythrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cell proliferation; positive regulation of granulocyte differentiation; positive regulation of macrophage differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of STAT protein; regulation of actin filament polymerization; regulation of transcription, DNA-dependent; response to hormone stimulus