Western Blot (WB)
(Western blot analysis (1) 200ng, (2) 100ng, (3) 50ng, (4) 25ng, and (5) 12.5ng of a HAT-tagged recombinant protein using biotinylated anti-HAT-tag monoclonal antibody at 0.5 μg/ml. )
Mouse HAT-tag Monoclonal Antibody
HAT-tag Antibody [4A6F3] (biotin)
Applications
ELISA, Western Blot
Purity
Affinity chromatography purified via peptide column.
Synonyms
HAT-tag; Monoclonal Antibody; HAT-tag Antibody [4A6F3] (biotin); Hat-tag; Hat; anti-HAT-tag antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
4A6F3
Specificity
HAT-tag antibody will detect amino- or carboxy-terminal HAT-tagged recombinant proteins.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid; HAT-tag antibody is supplied in PBS containing 1 % BSA ans 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Applicable Applications for anti-HAT-tag antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Western blot at 0.5 - 1u/ml.
Optimal dilutions for each application to be determine by the researcher.
Optimal dilutions for each application to be determine by the researcher.
Conjugate
Biotin
Immunogen
HAT-tag antibody was raised against a synthetic peptide containing KDHLIHNVHKEFHAHAHNK.
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis (1) 200ng, (2) 100ng, (3) 50ng, (4) 25ng, and (5) 12.5ng of a HAT-tagged recombinant protein using biotinylated anti-HAT-tag monoclonal antibody at 0.5 μg/ml. )
Western Blot (WB)
(Western blot analysis (1) 200ng, (2) 100ng, (3) 50ng, (4) 25ng, and (5) 12.5ng of a HAT-tagged recombinant protein using biotinylated anti-HAT-tag monoclonal antibody at 0.5 μg/ml. )
Related Product Information for anti-HAT-tag antibody
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. HAT-tag is one of the more popular epitope tags for expressed recombinant proteins.
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Product Notes
The HAT-tag (Catalog #AAA154520) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HAT-tag can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Western blot at 0.5 - 1u/ml. Optimal dilutions for each application to be determine by the researcher. Researchers should empirically determine the suitability of the HAT-tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HAT-tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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