Mouse anti-Human HAND1 Monoclonal Antibody | anti-HAND1 antibody

Mouse Monoclonal [clone 1G10] (IgG2b) to Human HAND1

Cat. Num. AAA249182

• Gene Names: HAND1; Hxt; eHand; Thing1; bHLHa27
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A/G Purified
• Synonyms: HAND1; Monoclonal Antibody; Mouse Monoclonal [clone 1G10] (IgG2b) to Human HAND1; Anti-HAND1 Antibody (clone 1G10) IHC-plus; EHand; Hxt; BHLHa27; Thing1; Human HAND1; anti-HAND1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 1G10
• Specificity: Human HAND1
• Purity/Purification: Protein A/G Purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Concentration: 0.82 mg/ml (varies by lot)
• Sequence Length: 215

• Applicable Applications for anti-HAND1 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: Flo (1:100), IF (1:100), IHC-P (10 ug/ml), WB (1:500)
• Target Species: Human
• Immunogen Description: Full length human recombinant protein of human HAND1 (NP_004812) produced in E Coli.
• Immunogen Type: Recombinant protein
• Immunogen: HAND1 antibody was raised against full length human recombinant protein of human HAND1 (NP_004812) produced in E Coli.
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-HAND1 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Anti-HAND1 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HAND1.)

Flow Cytometry (FC/FACS)

(Flow cytometry of HeLa cells, using anti-HAND1 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Flow Cytometry (FC/FACS)

(Flow cytometry of Jurkat cells, using anti-HAND1 antibody (Red), compared to a nonspecific negative control antibody (Blue).)

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HAND1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HAND1.)

Product Categories/Family for anti-HAND1 antibody

Family: Transcription factor

NCBI and Uniprot Product Information

• NCBI GI #: 4758506
• NCBI GeneID: 9421
• NCBI Accession #: NP_004812.1
• NCBI GenBank Nucleotide #: NM_004821.2
• UniProt Accession #: O96004
• Molecular Weight: 23,627 Da
• NCBI Official Full Name: heart- and neural crest derivatives-expressed protein 1
• NCBI Official Synonym Full Names: heart and neural crest derivatives expressed 1
• NCBI Official Symbol: HAND1
• NCBI Official Synonym Symbols: Hxt; eHand; Thing1; bHLHa27
• NCBI Protein Information: heart- and neural crest derivatives-expressed protein 1; class A basic helix-loop-helix protein 27; extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1
• UniProt Protein Name: Heart- and neural crest derivatives-expressed protein 1
• Protein Family: Heart- and neural crest derivatives-expressed protein
• UniProt Gene Name: HAND1
• UniProt Synonym Gene Names: BHLHA27; EHAND; bHLHa27; eHAND
• UniProt Entry Name: HAND1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, it has been suggested that this transcription factor may be required for early trophoblast differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

HAND1: Transcription factor that plays an essential role in both trophoblast-giant cells differentiation and in cardiac morphogenesis. In the adult, could be required for ongoing expression of cardiac-specific genes. Binds the DNA sequence 5'- NRTCTG-3' (non-canonical E-box).

Protein type: Transcription factor; DNA-binding; Nucleolus; Cell development/differentiation

Chromosomal Location of Human Ortholog: 5q33

Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus

Molecular Function: identical protein binding; protein binding; enzyme binding; protein homodimerization activity; protein heterodimerization activity; transcription coactivator activity; bHLH transcription factor binding; transcription factor binding; transcription corepressor activity

Biological Process: transcription from RNA polymerase II promoter; blastocyst development; negative regulation of transcription factor activity; heart development; negative regulation of transcription from RNA polymerase II promoter; trophectodermal cell differentiation; odontogenesis of dentine-containing teeth; mesoderm formation; embryonic heart tube development; ventricular cardiac muscle morphogenesis; positive regulation of transcription from RNA polymerase II promoter; heart looping; angiogenesis; negative regulation of transcription, DNA-dependent