Mouse anti-Human Guanylate Cyclase, Soluble Monoclonal Antibody | anti-Npr1 antibody

Guanylate Cyclase, Soluble (sGC)

Cat. Num. AAA609530

• Gene Names: Npr1; Gca; Npra; Anpra; NPR-A
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Guanylate Cyclase; Soluble; Monoclonal Antibody; Soluble (sGC); Anti -Guanylate Cyclase; anti-Npr1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 5E790
• Specificity: Recognizes hsGC b-subunit.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-Npr1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Immunogen: Recombinant human soluble guanylate cyclase a1b1 heterodimer.
• Hybridoma: Derived from hybridization of Sp 2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant human soluble guanylate cyclase a1b1 heterodimer.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degree C or colder. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-Npr1 antibody

Antibodies; Abs to Enzymes; Cyclase

NCBI and Uniprot Product Information

• NCBI GI #: 204270
• NCBI GeneID: 24603
• UniProt Accession #: P18910; A1A5N8
• Molecular Weight: 118,951 Da
• NCBI Official Full Name: guanylate cyclase
• NCBI Official Synonym Full Names: natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A)
• NCBI Official Symbol: Npr1
• NCBI Official Synonym Symbols: Gca; Npra; Anpra; NPR-A
• NCBI Protein Information: atrial natriuretic peptide receptor 1; GC-A; ANP-A; ANPR-A; guanylate cyclase A; atrial natriuretic peptide receptor A; atrial natriuretic peptide A-type receptor; atrial natriuretic peptide receptor type A
• UniProt Protein Name: Atrial natriuretic peptide receptor 1
• Protein Family: Regulatory protein
• UniProt Gene Name: Npr1
• UniProt Entry Name: ANPRA_RAT

NCBI Description

receptor for atrial natriuretic peptide [RGD]

Uniprot Description

ANPA: natriuretic peptide receptor A, which mediates natriuretic, diuretic, and vasorelaxing actions of the natriuretic peptides. Contains five functional domains: an extracellular ligand-binding domain, a single membrane-spanning region, and intracellularly a protein kinase homology domain, a helical hinge region involved in oligomerization, and a carboxyl-terminal guanylyl cyclase catalytic domain. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family. Has guanylate cyclase activity on binding of ANF. Hypertensive SHR Rat has polymorphism at the locus. Human non-coding polymorphisms can alter expression up to two-fold, and have been associated with hypertension. A coding SNP is associated with hypertension and myocardial infarction.

Protein type: Protein kinase, dual-specificity (receptor); Receptor, misc.; Kinase, protein; Lyase; Protein kinase, RGC; Guanylyl cyclase; Membrane protein, integral; EC 4.6.1.2; Nucleotide Metabolism - purine; RGC group; RGC family

Cellular Component: plasma membrane; integral to membrane; receptor complex

Molecular Function: protein binding; guanylate cyclase activity; GTP binding; hormone binding; natriuretic peptide receptor activity; peptide hormone binding; protein kinase binding; ATP binding; protein kinase activity

Biological Process: cGMP biosynthetic process; cell surface receptor linked signal transduction; positive regulation of cGMP biosynthetic process; negative regulation of smooth muscle cell proliferation; regulation of blood pressure; receptor guanylyl cyclase signaling pathway; regulation of blood vessel size; protein amino acid phosphorylation; dopamine metabolic process