Rabbit anti-Human RPL35A Polyclonal Antibody | anti-RPL35A antibody

RPL35A Rabbit pAb

Cat. Num. AAA4754542

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: RPL35A; Polyclonal Antibody; RPL35A Rabbit pAb; DBA5; L35A; anti-RPL35A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Purity/Purification: Affinity purified
• Form/Format: -20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

• Applicable Applications for anti-RPL35A antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: WB: 1:200-1:1000
• Immunogen: Full length fusion protein
• Conjugation: Unconjugated
• Molecular Function: Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome.
• Modification: Unmodified
• Preparation and Storage: Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-4: PC-3, 231, 293T and Hela cell, Primary antibody: 123663(RPL35A Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human brain tissue using 123663(RPL35A Antibody) at dilution 1/20. (Original magnification: ×200))

Product Categories/Family for anti-RPL35A antibody

Primary antibody

NCBI and Uniprot Product Information

• NCBI GI #: 34783080
• NCBI GeneID: 6165
• UniProt Accession #: P18077; Q08ES9; Q9BVN7
• Molecular Weight: 12,538 Da
• NCBI Official Full Name: Homo sapiens ribosomal protein L35a, mRNA
• UniProt Protein Name: 60S ribosomal protein L35a
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL35A
• UniProt Entry Name: RL35A_HUMAN

Uniprot Description

RPL35A: Required for the proliferation and viability of hematopoietic cells. Plays a role in 60S ribosomal subunit formation. The protein was found to bind to both initiator and elongator tRNAs and consequently was assigned to the P site or P and A site. Defects in RPL35A are the cause of Diamond-Blackfan anemia type 5 (DBA5). DBA5 is a form of Diamond- Blackfan anemia, a congenital non-regenerative hypoplastic anemia that usually presents early in infancy. Diamond-Blackfan anemia is characterized by a moderate to severe macrocytic anemia, erythroblastopenia, and an increased risk of malignancy. 30 to 40% of Diamond-Blackfan anemia patients present with short stature and congenital anomalies, the most frequent being craniofacial (Pierre-Robin syndrome and cleft palate), thumb and urogenital anomalies. Belongs to the ribosomal protein L35Ae family.

Protein type: RNA-binding; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 3q29

Cellular Component: membrane; mitochondrion; cytosol

Molecular Function: structural constituent of ribosome; tRNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; translation; viral reproduction; translational termination; viral infectious cycle; ribosomal large subunit biogenesis and assembly; cellular protein metabolic process; translational elongation; mRNA catabolic process, nonsense-mediated decay; translational initiation; viral transcription; gene expression; rRNA processing

Disease: Diamond-blackfan Anemia 5