Mouse anti-Human GSTA1 Monoclonal Antibody | anti-GSTA1 antibody

GSTA1 (Glutathione S-transferase A1, GST HA Subunit 1, GST Class-alpha Member 1, GST-epsilon, GSTA1-1, GTH1, MGC131939) (Biotin)

Cat. Num. AAA6142183

• Gene Names: GSTA1; GST2; GTH1; GSTA1-1; GST-epsilon
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography
• Synonyms: GSTA1; Monoclonal Antibody; GSTA1 (Glutathione S-transferase A1; GST HA Subunit 1; GST Class-alpha Member 1; GST-epsilon; GSTA1-1; GTH1; MGC131939) (Biotin); anti-GSTA1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2F7
• Specificity: Recognizes human GSTA1.
• Purity/Purification: Purified by Protein A Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 1176

• Applicable Applications for anti-GSTA1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-222 from human GSTA1 (AAH53578.1) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAEKPKLHYFNARGRMESTRWLLAAAGVEFEEKFIKSAEDLDKLRNDGYLMFQQVPMVEIDGMKLVQTRAILNYIASKYNLYGKDIKERALIDMYIEGIADLGEMILLLPVCPPEEKDAKLALIKEKIKNRYFPAFEKVLKSHGQDYLVGNKLSRADIHLVELLYYVEELDSSLISSFPLLKALKTRISNLPTVKKFLQPGSPRKPPMDEKSLEEARKIFRF
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (50.16kD).)

Western Blot (WB)

(Western Blot analysis of GSTA1 expression in transfected 293T cell line by GSTA1 monoclonal antibody. Lane 1: GSTA1 transfected lysate (26kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to GSTA1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged GSTA1 is ~0.1ng/ml as a capture antibody.)

Product Categories/Family for anti-GSTA1 antibody

Antibodies; Epitope Tag; GST

References

1. Redox Protein Expression Predicts Radiotherapeutic Response in Early-Stage Invasive Breast Cancer Patients. Woolston CM, Al-Attar A, Storr SJ, Ellis IO, Morgan DA, Martin SG.Int J Radiat Oncol Biol Phys. 2011 Feb 5. 2. Overcoming Glutathione S-Transferase P1-Related Cisplatin Resistance in Osteosarcoma. Pasello M, Michelacci F, Scionti I, Hattinger CM, Zuntini M, Caccuri AM, Scotlandi K, Picci P, Serra M.Cancer Res. 2008 Aug 15;68(16):6661-8.

NCBI and Uniprot Product Information

• NCBI GI #: 31657184
• NCBI GeneID: 2938
• NCBI Official Full Name: Homo sapiens glutathione S-transferase alpha 1, mRNA
• NCBI Official Synonym Full Names: glutathione S-transferase alpha 1
• NCBI Official Symbol: GSTA1
• NCBI Official Synonym Symbols: GST2; GTH1; GSTA1-1; GST-epsilon
• NCBI Protein Information: glutathione S-transferase A1
• Protein Family: Glutathione S-transferase

NCBI Description

This gene encodes a member of a family of enzymes that function to add glutathione to target electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. This action is an important step in detoxification of these compounds. This subfamily of enzymes has a particular role in protecting cells from reactive oxygen species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. This gene is located in a cluster of similar genes and pseudogenes on chromosome 6. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]