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Western Blot (WB) (ROPN1L Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the ROPN1L antibody detected the ROPN1L protein (arrow).)

Rabbit ROPN1L Polyclonal Antibody | anti-ROPN1L antibody

ROPN1L Antibody (N-term)

Gene Names
ROPN1L; ASP; RSPH11; LINC01513
Reactivity
Mouse (Predicted Reactivity: Bovine, Monkey)
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
ROPN1L; Polyclonal Antibody; ROPN1L Antibody (N-term); Ropporin-1-like protein; ROPN1-like protein; AKAP-associated sperm protein; ASP; anti-ROPN1L antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse (Predicted Reactivity: Bovine, Monkey)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This ROPN1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-60 amino acids from the N-terminal region of human ROPN1L.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.45 (varies by lot)
Sequence Positions
32-60
Sequence Length
230
Applicable Applications for anti-ROPN1L antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(ROPN1L Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the ROPN1L antibody detected the ROPN1L protein (arrow).)

Western Blot (WB) (ROPN1L Antibody (N-term) western blot analysis in mouse testis tissue lysates (35ug/lane).This demonstrates the ROPN1L antibody detected the ROPN1L protein (arrow).)

Immunohistochemistry (IHC)

(ROPN1L Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ROPN1L Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (ROPN1L Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ROPN1L Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-ROPN1L antibody
The protein encoded by this gene is a sperm protein, which
interacts with A-kinase anchoring protein, AKAP3, through the
amphipathic helix region of AKAP3. Type II regulatory subunit of
cAMP-dependent protein kinase (PKARII) also binds to this helix
domain of AKAP3, which allows PKARII to be targeted to specific
subcellular compartments. It is suggested that sperm contains
several proteins that bind to AKAPs in a manner similar to PKARII,
and this encoded protein may be one of them.
Product Categories/Family for anti-ROPN1L antibody
References
Carr, D.W., et al. J. Biol. Chem. 276(20):17332-17338(2001)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
26107
NCBI Official Full Name
ropporin-1-like protein
NCBI Official Synonym Full Names
rhophilin associated tail protein 1-like
NCBI Official Symbol
ROPN1L
NCBI Official Synonym Symbols
ASP; RSPH11; LINC01513
NCBI Protein Information
ropporin-1-like protein
UniProt Protein Name
Ropporin-1-like protein
Protein Family
UniProt Gene Name
ROPN1L
UniProt Synonym Gene Names
ASP; ROPN1-like protein
UniProt Entry Name
ROP1L_HUMAN

NCBI Description

This gene encodes a member of the ropporin family. The encoded protein is present in sperm and interacts with A-kinase anchoring protein, AKAP3, through the amphipathic helix region of AKAP3. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2014]

Uniprot Description

ROPN1L: a member of the ropporin family. The encoded protein is a sperm protein. It interacts with A-kinase anchoring protein, AKAP3, through the amphipathic helix region of AKAP3. Type II regulatory subunit of cAMP-dependent protein kinase (PKARII) also binds to this helix domain of AKAP3, which allows PKARII to be targeted to specific subcellular compartments. It is suggested that sperm contains several proteins that bind to AKAPs in a manner similar to PKARII, and this encoded protein may be one of them. Alternatively spliced transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2011]

Protein type: Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 5p15.2

Cellular Component: cytoplasm

Molecular Function: protein binding

Biological Process: sperm motility; regulation of protein amino acid phosphorylation; sperm capacitation

Research Articles on ROPN1L

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Product Notes

The ROPN1L ropn1l (Catalog #AAA9201163) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 32-60. The ROPN1L Antibody (N-term) reacts with Mouse (Predicted Reactivity: Bovine, Monkey) and may cross-react with other species as described in the data sheet. AAA Biotech's ROPN1L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the ROPN1L ropn1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ROPN1L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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