Mouse anti-Human GPI Monoclonal Antibody | anti-GPI antibody

Mouse Monoclonal [clone 1B7D7] (IgG1) to Human GPI

Cat. Num. AAA246581

• Gene Names: GPI; AMF; NLK; PGI; PHI; GNPI; SA36; SA-36
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Ascites
• Synonyms: GPI; Monoclonal Antibody; Mouse Monoclonal [clone 1B7D7] (IgG1) to Human GPI; Anti-GPI Antibody (clone 1B7D7) IHC-plus; AMF; Autocrine motility factor; Glucose phosphate isomerase; Hexose monophosphate isomerase; Hexosephosphate isomerase; Neuroleukin; Oxoisomerase; Phosphoglucose isomerase; Phosphohexomutase; Phosphohexose isomerase; SA36; PGI; SA-36; Human GPI; anti-GPI antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1B7D7
• Specificity: Human GPI
• Purity/Purification: Ascites
• Form/Format: Ascites fluid, 0.03% sodium azide.
• Sequence Length: 558

• Applicable Applications for anti-GPI antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC-P (1:200); IF (1:200 - 1:1000); WB (1:100 - 1:500); ELISA (1:10000); ; Optimal dilution to be determined by the researcher.
• Target Species: Human
• Immunogen Description: Purified recombinant fragment of human GPI expressed in E Coli.
• Immunogen: GPI antibody was raised against purified recombinant fragment of human GPI expressed in E Coli.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Note: During shipment,small volumes of product will ocassionally become entrapped in the seal of the product vial. We recommend briefly centrifuging the vial to dislodge any liquid in the container's cap prior to opening.
• Warning: This reagent may contain sodium azide. The chemical, physical, and toxicologial properties of this material have not been thoughly investigated.Standard Laboratory Practices should be followed. Avoid skin and eye contact,inhalation, and ingestion. Sodium azide forms hydrazoic acid under acidic conditions and may react with lead or copper plubing to form highly explosive metal azides. On disposal, flush with large volumes of water to prevent accumulation.
• Preparation and Storage: Short term: 4°C .; Long term: -20°C

Immunohistochemistry (IHC)

(Anti-GPI antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

Immunohistochemistry (IHC)

(Anti-GPI antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.)

Immunofluorescence (IF)

(Confocal immunofluorescence of L-02 cells using anti-GPI mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.)

Western Blot (WB)

(Western blot of anti-GPI mAb against HepG2 (1), SMMC-7721 (2) cell lysate and rat liver tissues lysate (3).)

NCBI and Uniprot Product Information

• NCBI GI #: 18201905
• NCBI GeneID: 2821
• NCBI Accession #: NP_000166.2
• NCBI GenBank Nucleotide #: NM_000175.3
• UniProt Accession #: P06744; Q9BRD3; Q9BSK5; Q9UHE6; B4DG39
• NCBI Official Full Name: glucose-6-phosphate isomerase isoform 2
• NCBI Official Synonym Full Names: glucose-6-phosphate isomerase
• NCBI Official Symbol: GPI
• NCBI Official Synonym Symbols: AMF; NLK; PGI; PHI; GNPI; SA36; SA-36
• NCBI Protein Information: glucose-6-phosphate isomerase; neuroleukin; oxoisomerase; sperm antigen 36; sperm antigen-36; phosphohexomutase; phosphosaccharomutase; phosphohexose isomerase; phosphoglucose isomerase; autocrine motility factor; hexosephosphate isomerase; glucose phosphate isomerase; hexose monophosphate isomerase
• UniProt Protein Name: Glucose-6-phosphate isomerase
• Protein Family: GPI-anchored protein
• UniProt Gene Name: GPI
• UniProt Synonym Gene Names: GPI; AMF; NLK; PGI; PHI; SA-36
• UniProt Entry Name: G6PI_HUMAN

NCBI Description

This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

G6PI: belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. A dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Protein type: EC 5.3.1.9; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - starch and sucrose; Apoptosis; Cytokine; Isomerase

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: nucleoplasm; extracellular space; neuron projection; membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: monosaccharide binding; glucose-6-phosphate isomerase activity; growth factor activity; cytokine activity; intramolecular transferase activity

Biological Process: aldehyde catabolic process; methylglyoxal biosynthetic process; glycolysis; learning and/or memory; hemostasis; glucose 6-phosphate metabolic process; carbohydrate metabolic process; glucose metabolic process; pathogenesis; negative regulation of neuron apoptosis; angiogenesis; negative regulation of caspase activity; humoral immune response; gluconeogenesis

Disease: Hemolytic Anemia, Nonspherocytic, Due To Glucose Phosphate Isomerase Deficiency