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Immunohistochemistry (IHC) (Immunohistochemistry analysis of human breast cancer tissue immunohistochemically stained using Grp75 mAb (30A5).)

Mouse Grp75/Mortalin Monoclonal Antibody | anti-MtHsp70; Hsp70 (mitochondrial); HspA9 antibody

Grp75/Mortalin monoclonal antibody (30A5)

Gene Names
HSPA9; CSA; MOT; MOT2; SAAN; CRP40; EVPLS; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75; HEL-S-124m
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Drosophila, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
Applications
Immunohistochemistry, Western Blot
Purity
Ammonium sulfate precipitation purified.
Synonyms
Grp75/Mortalin; Monoclonal Antibody; Grp75/Mortalin monoclonal antibody (30A5); anti-MtHsp70; Hsp70 (mitochondrial); HspA9 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Drosophila, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus
Clonality
Monoclonal
Isotype
IgG1
Clone Number
30A5
Specificity
Recognizes human, mouse, rat, bovine, dog, chicken, Drosophila, guinea pig, hamster, monkey, pig, rabbit, sheep and Xenopus Grp75. Detects a band of ~75kDa by Western blot.
Purity/Purification
Ammonium sulfate precipitation purified.
Form/Format
Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
Sequence Length
679
Applicable Applications for anti-MtHsp70; Hsp70 (mitochondrial); HspA9 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Immunohistochemistry (1:50)
Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~75kDa by Western blot.
Immunogen
Native human Grp75 (Mortalin).
Preparation and Storage
Long term storage: -20 degree C
Avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of human breast cancer tissue immunohistochemically stained using Grp75 mAb (30A5).)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of human breast cancer tissue immunohistochemically stained using Grp75 mAb (30A5).)
Related Product Information for anti-MtHsp70; Hsp70 (mitochondrial); HspA9 antibody
Grp75 (Glucose regulated 75 kDa protein) is a member of the Hsp70 family. It is localized in the mitochondrial matrix, where, in concert with Hsp60, is thought to participate in the re-folding of proteins translocated into this organelle. Like its E Coli homolog DnaK, Grp75 possesses a cation-dependent ATPase activity thought to be central to its function as a chaperone.
Product Categories/Family for anti-MtHsp70; Hsp70 (mitochondrial); HspA9 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
73,680 Da
NCBI Official Full Name
Stress-70 protein, mitochondrial
NCBI Official Synonym Full Names
heat shock protein family A (Hsp70) member 9
NCBI Official Symbol
HSPA9
NCBI Official Synonym Symbols
CSA; MOT; MOT2; SAAN; CRP40; EVPLS; GRP75; PBP74; GRP-75; HSPA9B; MTHSP75; HEL-S-124m
NCBI Protein Information
stress-70 protein, mitochondrial
UniProt Protein Name
Stress-70 protein, mitochondrial
UniProt Gene Name
HSPA9
UniProt Synonym Gene Names
GRP75; HSPA9B; mt-HSP70; GRP-75; MOT; PBP74
UniProt Entry Name
GRP75_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.[provided by RefSeq, May 2010]

Uniprot Description

HSPA9B: Implicated in the control of cell proliferation and cellular aging. May also act as a chaperone. Interacts with FXN. Interacts with HSCB. Component of the MINOS/MitOS complex, that includes IMMT, HSPA9 and CHCHD3 and associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2. Belongs to the heat shock protein 70 family.

Protein type: Chaperone; Nucleolus; Heat shock protein

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: cytoplasm; focal adhesion; mitochondrion; myelin sheath; nucleolus

Molecular Function: ATP binding; fibroblast growth factor binding; heat shock protein binding; protein binding; ubiquitin protein ligase binding; unfolded protein binding

Biological Process: cellular protein metabolic process; negative regulation of apoptosis; protein export from nucleus; protein folding; protein targeting to mitochondrion; response to toxin

Research Articles on MtHsp70; Hsp70 (mitochondrial); HspA9

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Product Notes

The MtHsp70; Hsp70 (mitochondrial); HspA9 hspa9 (Catalog #AAA565085) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Grp75/Mortalin monoclonal antibody (30A5) reacts with Human, Mouse, Rat, Bovine, Chicken, Dog, Drosophila, Guinea pig, Hamster, Monkey, Porcine, Rabbit, Sheep, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's Grp75/Mortalin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Immunohistochemistry (1:50) Western Blot (1:1,000, colorimetric) Suggested dilutions/conditions may not be available for all applications. Detects a band of ~75kDa by Western blot. Researchers should empirically determine the suitability of the MtHsp70; Hsp70 (mitochondrial); HspA9 hspa9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Grp75/Mortalin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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