Mouse anti-Human Glucokinase Monoclonal Antibody | anti-GCK antibody

Anti-Glucokinase Antibody, Mouse Monoclonal

Cat. Num. AAA8104817

• Gene Names: GCK; GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3
• Reactivity: Human
• Applications: ELISA
• Purity: Protein A
• Synonyms: Glucokinase; Monoclonal Antibody; Anti-Glucokinase Antibody; Mouse Monoclonal; Glucokinase Antibody; Mouse MAb; glucokinase (hexokinase 4); Anti-FGQTL3 Antibody; Anti-GK Antibody; Anti-GLK Antibody; Anti-HHF3 Antibody; Anti-HK4 Antibody; Anti-HKIV Antibody; Anti-HXKP Antibody; Anti-LGLK Antibody; Anti-MODY2 Antibody; anti-GCK antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 13
• Specificity: Human Glucokinase
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GCK antibody: ELISA (EIA)
• Application Notes: ELISA: 1:1000-1:2000
• Immunogen: Recombinant Human Glucokinase Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Glucokinase (rh Glucokinase). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-GCK antibody

Glucokinase belongs to the bacterial glucokinase family. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). It can Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Glucokinase has a high Km for glucose, and so it is effective only when glucose is abundant. The role of GCK is to provide G6P for the synthesis of glycogen. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage. It has a pivotal role as glucose sensor of the pancreatic beta-cells. Glucokinase explains the capacity, hexose specificity, affinities, sigmoidicity, and anomeric preference of pancreatic islet glycolysis, and because stimulation of glucose metabolism is a prerequisite of glucose stimulation of insulin release, glucokinase also explains many characteristics of this beta-cell function. Glucokinase of the beta-cell is induced or activated by glucose in contrast to liver glucokinase, which is regulated by insulin. Tissue-specific regulation corresponds with observations that liver and pancreatic beta-cell glucokinase are structurally distinct. Glucokinase could play a glucose-sensor role in hepatocytes as well, and certain forms of diabetes mellitus might be due to glucokinase deficiencies in pancreatic beta-cells, hepatocytes, or both.

Product Categories/Family for anti-GCK antibody

Death Receptor Signaling

References

Matschinsky FM. (1990) Glucokinase as glucose sensor and metabolic signal generator in pancreatic beta-cells and hepatocytes. Diabetes. 39(6): 647-52.Magnuson MA, et al. (2004) Glucokinase as a glucose sensor: past, present, and future. Glucokinase And Glycemic Disease: From Basics to Novel Therapeutics (Frontiers in Diabetes). Basel: S. Karger AG (Switzerland). pp. 18-30.Cardenas ML. (2004) Comparative biochemistry of glucokinase. Glucokinase And Glycemic Disease: From Basics to Novel Therapeutics (Frontiers in Diabetes). Basel: S. Karger AG (Switzerland). pp. 31-41.

NCBI and Uniprot Product Information

• NCBI GI #: 4503951
• NCBI GeneID: 2645
• NCBI Accession #: NP_000153.1
• NCBI GenBank Nucleotide #: NM_000162.3
• UniProt Accession #: P35557; Q05810; A4D2J2; A4D2J3
• Molecular Weight: 52,035 Da
• NCBI Official Full Name: glucokinase isoform 1
• NCBI Official Synonym Full Names: glucokinase (hexokinase 4)
• NCBI Official Symbol: GCK
• NCBI Official Synonym Symbols: GK; GLK; HK4; HHF3; HKIV; HXKP; LGLK; MODY2; FGQTL3
• NCBI Protein Information: glucokinase; ATP:D-hexose 6-phosphotransferase; HK IV; hexokinase D, pancreatic isozyme; hexokinase type IV; hexokinase-4; hexokinase-D
• UniProt Protein Name: Glucokinase
• Protein Family: Glucokinase
• UniProt Gene Name: GCK
• UniProt Synonym Gene Names: HK IV; HK4
• UniProt Entry Name: HXK4_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI). [provided by RefSeq, Apr 2009]