Rabbit GluR1 Polyclonal Antibody | anti-GluR1 antibody

GluR1 (S857) Polyclonal Antibody

Cat. Num. AAA3004090

• Gene Names: GRIA1; GLUH1; GLUR1; GLURA; GluA1; HBGR1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GluR1; Polyclonal Antibody; GluR1 (S857) Polyclonal Antibody; Glutamate receptor 1; GluR-1; AMPA-selective glutamate receptor 1; GluR-A; GluR-K1; GluRA; GluRK1; Glutamate receptor ionotropic; AMPA 1; GluA1; GRIA1; GLUH1; GLUR1; anti-GluR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GluR1 (S857) polyclonal antibody detects endogenous levels of GluR1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 906

• Applicable Applications for anti-GluR1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 830-880 of Human GluR1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of GluR1 (S857) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.showing cell membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-GluR1 antibody

Glutamate receptors mediate most excitatory neurotransmission in the brain and play an important role in neural plasticity, neural development and neurodegeneration. Ionotropic glutamate receptors are categorized into NMDA receptors and kainate/AMPA receptors, both of which contain glutamate-gated, cation-specific ion channels. Kainate/AMPA receptors are co-localized with NMDA receptors in many synapses and consist of seven structurally related subunits designated GluR-1 to -7. The kainate/AMPA receptors are primarily responsible for the fast excitatory neuro-transmission by glutamate whereas the NMDA receptors are functionally characterized by a slow kinetic and a high permeability for Ca2+ ions.

NCBI and Uniprot Product Information

• NCBI GI #: 167001419
• NCBI GeneID: 2890
• NCBI Accession #: NP_000818.2
• NCBI GenBank Nucleotide #: NP_000818.2
• UniProt Accession #: P42261
• Molecular Weight: ~ 102kDa
• NCBI Official Full Name: glutamate receptor 1 isoform 1
• NCBI Official Synonym Full Names: glutamate ionotropic receptor AMPA type subunit 1
• NCBI Official Symbol: GRIA1
• NCBI Official Synonym Symbols: GLUH1; GLUR1; GLURA; GluA1; HBGR1
• NCBI Protein Information: glutamate receptor 1
• UniProt Protein Name: Glutamate receptor 1
• UniProt Gene Name: GRIA1
• UniProt Synonym Gene Names: GLUH1; GLUR1; GluR-1; GluA1
• UniProt Entry Name: GRIA1_HUMAN

NCBI Description

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GluR1: an integral membrane protein belonging to the glutamate-gated ion channel family. L-glutamate (Glu) acts as an excitatory neurotransmitter at many synapses in the central nervous system. Glutamate receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. Each of the four GluR proteins (GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. Expressed in granule and pyramidal cells in the hippocampal formation.

Protein type: Membrane protein, integral; Channel, ligand-gated; Channel, calcium; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: postsynaptic membrane; synaptic vesicle; endoplasmic reticulum membrane; recycling endosome; cell surface; cell soma; postsynaptic density; dendrite; dendritic spine; plasma membrane; cell junction

Molecular Function: protein binding; extracellular-glutamate-gated ion channel activity; alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity; glutamate receptor activity; PDZ domain binding

Biological Process: synaptic transmission, glutamatergic; synaptic transmission; long-term memory; ionotropic glutamate receptor signaling pathway; receptor internalization; signal transduction