Rabbit anti-Human HBQ1 Polyclonal Antibody | anti-HBQ1 antibody

HBQ1, NT (HBQ1, Hemoglobin subunit theta-1, Hemoglobin theta-1 chain, Theta-1-globin) (FITC)

Cat. Num. AAA6305628

• Gene Names: HBQ1; HBQ
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HBQ1; Polyclonal Antibody; NT (HBQ1; Hemoglobin subunit theta-1; Hemoglobin theta-1 chain; Theta-1-globin) (FITC); Theta-1-globin; anti-HBQ1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-HBQ1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: HBQ1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HBQ1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(HBQ1 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the HBQ1 antibody detected the HBQ1 protein (arrow).)

Immunohistochemistry (IHC)

(HBQ1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HBQ1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-HBQ1 antibody

Antibodies; Hemoglobin

References

De Gobbi, M., et al. Science 312(5777):1215-1217(2006). Patrinos, G.P., et al. Hum. Mutat. 26(5):399-412(2005). Daniels, R.J., et al. Hum. Mol. Genet. 10(4):339-352(2001). Giardina, B., et al. Crit. Rev. Biochem. Mol. Biol. 30(3):165-196(1995) Kim, J.H., et al. Nucleic Acids Res. 17(14):5687-5700(1989).

NCBI and Uniprot Product Information

• NCBI GI #: 4885395
• NCBI GeneID: 3049
• NCBI Accession #: NP_005322.1
• NCBI GenBank Nucleotide #: NM_005331.4
• UniProt Accession #: P09105; Q13723; Q1W6G5
• Molecular Weight: 15,508 Da
• NCBI Official Full Name: hemoglobin subunit theta-1
• NCBI Official Synonym Full Names: hemoglobin, theta 1
• NCBI Official Symbol: HBQ1
• NCBI Official Synonym Symbols: HBQ
• NCBI Protein Information: hemoglobin subunit theta-1; hemoglobin theta-1 chain; theta-1-globin
• UniProt Protein Name: Hemoglobin subunit theta-1
• Protein Family: Hemoglobin
• UniProt Gene Name: HBQ1
• UniProt Entry Name: HBAT_HUMAN

NCBI Description

Theta-globin mRNA is found in human fetal erythroid tissue but not in adult erythroid or other nonerythroid tissue. The theta-1 gene may be expressed very early in embryonic life, perhaps sometime before 5 weeks. Theta-1 is a member of the human alpha-globin gene cluster that involves five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-2 -pseudoalpha-1 - alpha-2 - alpha-1 - theta-1 - 3'. Research supports a transcriptionally active role for the gene and a functional role for the peptide in specific cells, possibly those of early erythroid tissue. [provided by RefSeq, Jul 2008]

Uniprot Description

HBQ1: Theta-globin mRNA is found in human fetal erythroid tissue but not in adult erythroid or other nonerythroid tissue. The theta-1 gene may be expressed very early in embryonic life, perhaps sometime before 5 weeks. Theta-1 is a member of the human alpha-globin gene cluster that involves five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-2 -pseudoalpha-1 - alpha-2 - alpha-1 - theta-1 - 3'. Research supports a transcriptionally active role for the gene and a functional role for the peptide in specific cells, possibly those of early erythroid tissue. [provided by RefSeq, Jul 2008]

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: hemoglobin complex

Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: oxygen transport