Rabbit anti-Rat GFRA1  Monoclonal Antibody | anti-GFRA1  antibody

Rabbit anti Rat GFRA1  Monoclonal Antibody

Cat. Num. AAA2544694

• Gene Names: GFRA1; GDNFR; RET1L; RETL1; TRNR1; GDNFRA; GFR-ALPHA-1
• Reactivity: Rat
• Applications: ELISA
• Synonyms: GFRA1 ; Monoclonal Antibody; Rabbit anti Rat GFRA1 Monoclonal Antibody; anti-GFRA1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 465

• Applicable Applications for anti-GFRA1 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Rat GFRA1. The detection limit for Rat GFRA1 is approximately 0.039 ng/well.
• Immunogen: Recombinant Rat GFRA1 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-GFRA1  antibody

Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 1 (GFRA1) is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA1 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA/GDNFRa acts as the ligand-binding component. GDNF, a distantly related member of the transforming growth factor-beta (TGF-) superfamily, and its receptor components: GFRA1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells.

NCBI and Uniprot Product Information

• NCBI GI #: 1129371469
• NCBI GeneID: 2674
• NCBI GenBank Nucleotide #: NM_001348098.1
• Molecular Weight: 50,838 Da
• NCBI Official Full Name: GDNF family receptor alpha-1 isoform a preproprotein
• NCBI Official Synonym Full Names: GDNF family receptor alpha 1
• NCBI Official Symbol: GFRA1
• NCBI Official Synonym Symbols: GDNFR; RET1L; RETL1; TRNR1; GDNFRA; GFR-ALPHA-1
• NCBI Protein Information: GDNF family receptor alpha-1
• UniProt Protein Name: GDNF family receptor alpha-1
• UniProt Gene Name: GFRA1
• UniProt Synonym Gene Names: GDNFRA; RETL1; TRNR1; GDNF receptor alpha-1; GDNFR-alpha-1; GFR-alpha-1
• UniProt Entry Name: GFRA1_HUMAN

NCBI Description

This gene encodes a member of the glial cell line-derived neurotrophic factor receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature receptor. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. This receptor is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]

Uniprot Description

GFRA1: Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor. Belongs to the GDNFR family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Receptor, misc.

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: extrinsic to membrane; plasma membrane

Molecular Function: glial cell line-derived neurotrophic factor receptor activity; Ras guanyl-nucleotide exchange factor activity; receptor binding

Biological Process: MAPKKK cascade