Rabbit anti-Human Ferritin Heavy Chain / FTH1 Polyclonal Antibody | anti-FTH1 antibody

Rabbit Polyclonal to Human Ferritin Heavy Chain / FTH1

Cat. Num. AAA248317

• Gene Names: FTH1; FHC; FTH; HFE5; PLIF; FTHL6; PIG15
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A purified
• Synonyms: Ferritin Heavy Chain / FTH1; Polyclonal Antibody; Rabbit Polyclonal to Human Ferritin Heavy Chain / FTH1; Anti-Ferritin Heavy Chain / FTH1 Antibody (aa154-183) IHC-plus; FTH1; Apoferritin; Ferritin H subunit; Ferritin heavy chain; Ferritin; heavy polypeptide 1; FHC; FTHL6; PIG15; PLIF; FTH; Human Ferritin Heavy Chain; anti-FTH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: This FTH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154-183 amino acids from the C-terminal region of human FTH1.
• Purity/Purification: Protein A purified
• Form/Format: PBS, 0.09% sodium azide
• Sequence Length: 154-183

• Applicable Applications for anti-FTH1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), Flow Cytometry (FC/FACS)
• Application Notes: IHC - Paraffin (1:100); Western blot (1:1000); Flow Cytometry (1:10 - 1:50); (Optimal dilution to be determined by the researcher)
• Target: FTH1 / Ferritin Heavy chain
• Immunogen Species: FTH1 / Ferritin Heavy chain antibody was raised against Human
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-Ferritin Heavy Chain / FTH1 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)

Flow Cytometry (FC/FACS)

(Flow cytometry of HepG2 cells using FTH1 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot of FTH1 Antibody (C-term) in A2058 cell line lysates (35 ug/lane). FTH1 (arrow) was detected using the purified Pab.)

NCBI and Uniprot Product Information

• NCBI GI #: 56682959
• NCBI GeneID: 2495
• NCBI Accession #: NP_002023.2
• NCBI GenBank Nucleotide #: NM_002032.2
• UniProt Accession #: P02794; Q3KRA8; Q3SWW1; B3KNR5
• NCBI Official Full Name: ferritin heavy chain
• NCBI Official Synonym Full Names: ferritin, heavy polypeptide 1
• NCBI Official Symbol: FTH1
• NCBI Official Synonym Symbols: FHC; FTH; HFE5; PLIF; FTHL6; PIG15
• NCBI Protein Information: ferritin heavy chain; apoferritin; ferritin H subunit; placenta immunoregulatory factor; proliferation-inducing protein 15; cell proliferation-inducing gene 15 protein
• UniProt Protein Name: Ferritin heavy chain
• Protein Family: Iron transporter
• UniProt Gene Name: FTH1
• UniProt Synonym Gene Names: FTH; FTHL6; Ferritin H subunit
• UniProt Entry Name: FRIH_HUMAN

NCBI Description

This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Ferritin-H: Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney. Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited. Belongs to the ferritin family.

Protein type: Oxidoreductase; EC 1.16.3.1; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: ferritin complex; mitochondrion; nucleus; cytosol

Molecular Function: ferroxidase activity; protein binding; ferric iron binding; iron ion binding

Biological Process: negative regulation of cell proliferation; receptor-mediated endocytosis; cellular iron ion homeostasis; negative regulation of fibroblast proliferation; immune response; post-Golgi vesicle-mediated transport; transmembrane transport; iron ion transport; intracellular sequestering of iron ion

Disease: Hemochromatosis, Type 5