Mouse Geminin Monoclonal Antibody | anti-GMNN antibody

Mouse Anti Human Geminin

Cat. Num. AAA140178

• Gene Names: GMNN; Gem
• Applications: ELISA, Western Blot
• Purity: Geminin antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Geminin; Monoclonal Antibody; Mouse Anti Human Geminin; GMNN Antibody; Mouse Anti Human; GMNN; DNA Replication Inhibitor; Gem; RP3-369A17.3; anti-GMNN antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 heavy chain and kappa light chain
• Clone Number: P2H7AT
• Purity/Purification: Geminin antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Sequence Length: 209

• Applicable Applications for anti-GMNN antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Geminin antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000. Recommended starting dilution is 1:500.
• Immunogen: Anti-human Geminin mAb is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Geminin amino acids 1-209 purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-GMNN antibody

Introduction: Geminin is a 25 kDa nuclear protein, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. Geminin controls replication by binding to the licensing factor Cdt1, and is involved in neural differentiation. In addition, Geminin directly interacts with Six3 and Hox homeodomain proteins during embryogenesis and inhibits their functions. Geminin can also promote DNA replication. Geminin has 2 roles in 2 different stages of the cell cycle: Geminin is a negative regulator of DNA replication during the œS phase of the cell cycle. Inhibition of Geminin during the œS phase (by RNAi) results in an additional round of replication of portions of the genome. During the œM phase of the cell cycle (mitosis) Geminin stabilizes the replication factor Cdt1 promoting DNA replication during the next cell cycle. Moreover, inhibition of Geminin during mitosis (by RNAi) causes destabilization of Cdt1 protein and impairment of DNA replication during the next cell cycle. G

Product Categories/Family for anti-GMNN antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 354681980
• NCBI GeneID: 51053
• NCBI Accession #: NP_001238918.1
• NCBI GenBank Nucleotide #: NM_001251989.1
• Molecular Weight: 23,565 Da
• NCBI Official Full Name: geminin
• NCBI Official Synonym Full Names: geminin, DNA replication inhibitor
• NCBI Official Symbol: GMNN
• NCBI Official Synonym Symbols: Gem
• NCBI Protein Information: geminin
• UniProt Protein Name: Geminin
• Protein Family: Geminin
• UniProt Gene Name: GMNN
• UniProt Entry Name: GEMI_HUMAN

NCBI Description

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011]

Uniprot Description

Geminin: Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Homotetramer. Interacts with CDT1; inhibits binding of the MCM complex to origins of replication. Interacts with IDAS; targets GMNN to the nucleus, prevents GMNN interaction with CDT1 and competes with IDAS homodimerization. Belongs to the geminin family.

Protein type: Cell cycle regulation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; histone deacetylase binding; transcription corepressor activity

Biological Process: organ morphogenesis; negative regulation of DNA replication; protein complex assembly; mitotic cell cycle; negative regulation of transcription, DNA-dependent; negative regulation of cell cycle