Rabbit anti-Human Geminin Polyclonal Antibody | anti-GMNN antibody

Geminin

Cat. Num. AAA623260

• Gene Names: GMNN; Gem; RP3-369A17.3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Geminin; Polyclonal Antibody; Anti -Geminin; anti-GMNN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of human geminin protein.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4, 0.02% sodium azide.

• Applicable Applications for anti-GMNN antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 0.05-0.2ug/ml; Western Blot: 0.5-1ug/ml
• Immunogen: Synthetic peptide within residues 50-100 of human geminin protein (Swiss Prot: O75496).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Lane 1: Hela cell lysate Lane 2: HEK293 cell lysate Lane 3: Recombinant human Geminin protein Western blot analysis of cell lysates and recombinant human Geminin protein using 1ug/ml MBS623260. The signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG.)

Related Product Information for anti-GMNN antibody

Geminin inhibits DNA replication by preventing the incorporation of the MCM complex into the prereplication complex (pre-RC). It is absent during the G1 phase, accumulates during the S, G2, and M phases, and disappears at the time of the metaphase-anaphase transition. Geminin contains 212 amino acids and has a destruction box sequence of RRTLKVIQP. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

Product Categories/Family for anti-GMNN antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 7705682
• NCBI GeneID: 51053
• NCBI Accession #: NP_056979.1
• NCBI GenBank Nucleotide #: NM_015895.3
• UniProt Accession #: O75496; Q9H1Z1; B3KMM8; Q53GD9
• Molecular Weight: 23,565 Da
• NCBI Official Full Name: geminin
• NCBI Official Synonym Full Names: geminin, DNA replication inhibitor
• NCBI Official Symbol: GMNN
• NCBI Official Synonym Symbols: Gem; RP3-369A17.3
• NCBI Protein Information: geminin; OTTHUMP00000016095; OTTHUMP00000016096; OTTHUMP00000016097
• UniProt Protein Name: Geminin
• Protein Family: Geminin
• UniProt Gene Name: GMNN
• UniProt Entry Name: GEMI_HUMAN

NCBI Description

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011]

Uniprot Description

Geminin: Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Homotetramer. Interacts with CDT1; inhibits binding of the MCM complex to origins of replication. Interacts with IDAS; targets GMNN to the nucleus, prevents GMNN interaction with CDT1 and competes with IDAS homodimerization. Belongs to the geminin family.

Protein type: Cell cycle regulation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; histone deacetylase binding; transcription corepressor activity

Biological Process: organ morphogenesis; negative regulation of DNA replication; protein complex assembly; mitotic cell cycle; negative regulation of transcription, DNA-dependent; negative regulation of cell cycle