Western Blot (WB)
(Figure 1: Western blot analysis using NR6A1 mAb against human NR6A1 recombinant protein. (Expected MW is 32.4 kDa))
Mouse anti-Human NR6A1 Monoclonal Antibody | anti-NR6A1 antibody
NR6A1 Antibody
Gene Names
NR6A1; RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA, Flow Cytometry, Functional Assay
Purity
Affinity-Chromatography
Synonyms
NR6A1; Monoclonal Antibody; NR6A1 Antibody; GCNF 1; GCNF; GCNF1; Germ cell nuclear factor; hGCNF; hRTR; NR 61; NR61; nr6a1; NR6A1_HUMAN; Nuclear receptor subfamily 6 group A member 1; Orphan nuclear receptor NR6A1; Retinoic acid receptor related testis associated receptor; Retinoid receptor related testis specific receptor; Retinoid receptor-related testis-specific receptor; RTR; anti-NR6A1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
NR6A1 antibody detects endogenous levels of total NR6A1
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
476
Applicable Applications for anti-NR6A1 antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
ICC: 1:200-1:1000
FC/FACS: 1:200-1:400
WB: 1:500-1:2000
IHC: 1:200-1:1000
ICC: 1:200-1:1000
FC/FACS: 1:200-1:400
Immunogen
Purified recombinant fragment of human NR6A1 expressed in E. Coli
Subcellular Location
Nucleus.
Tissue Specificity
Shows highest expression in the germ cells of the adult testis.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using NR6A1 mAb against human NR6A1 recombinant protein. (Expected MW is 32.4 kDa))
Western Blot (WB)
(Figure 1: Western blot analysis using NR6A1 mAb against human NR6A1 recombinant protein. (Expected MW is 32.4 kDa))
Related Product Information for anti-NR6A1 antibody
Description: This gene encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified.
Function: Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis (By similarity).
Subunit Structure: Homodimer. Interacts with UIMC1 (By similarity).
Similarity: Belongs to the nuclear hormone receptor family. NR6 subfamily.
Function: Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis (By similarity).
Subunit Structure: Homodimer. Interacts with UIMC1 (By similarity).
Similarity: Belongs to the nuclear hormone receptor family. NR6 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 54.3 kDa
Predicted: 55 kDa
Predicted: 55 kDa
NCBI Official Full Name
nuclear receptor subfamily 6 group A member 1 isoform 4
NCBI Official Synonym Full Names
nuclear receptor subfamily 6 group A member 1
NCBI Official Symbol
NR6A1
NCBI Official Synonym Symbols
RTR; GCNF; NR61; hRTR; CT150; GCNF1; hGCNF
NCBI Protein Information
nuclear receptor subfamily 6 group A member 1
UniProt Protein Name
Nuclear receptor subfamily 6 group A member 1
Protein Family
UniProt Gene Name
NR6A1
UniProt Synonym Gene Names
GCNF; GCNF; hGCNF; RTR; hRTR
NCBI Description
This gene encodes an orphan nuclear receptor which is a member of the nuclear hormone receptor family. Its expression pattern suggests that it may be involved in neurogenesis and germ cell development. The protein can homodimerize and bind DNA, but in vivo targets have not been identified. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Jun 2013]
Uniprot Description
Orphan nuclear receptor. Binds to a response element containing the sequence 5'-TCAAGGTCA-3'. May be involved in the regulation of gene expression in germ cell development during gametogenesis ().
Research Articles on NR6A1
Similar Products
Product Notes
The NR6A1 nr6a1 (Catalog #AAA9602329) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NR6A1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NR6A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:10000 WB: 1:500-1:2000 IHC: 1:200-1:1000 ICC: 1:200-1:1000 FC/FACS: 1:200-1:400. Researchers should empirically determine the suitability of the NR6A1 nr6a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NR6A1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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