Western Blot (WB)
(All lanes: Mouse anti-GAPDH Monoclonal antibody at 1ug/mlLane 1:A549 whole cell lysateLane 2:HT-29 whole cell lysateLane 3:Jurkat whole cell lysateLane 4:U251 whole cell lysateLane 5:COLO205 whole cell lysateLane 6:MCF7 whole cell lysateLane 7:K562 whole cell lysateLane 8:Hela whole cell lysateLane 9:293T whole cell lysateSecondaryGoat polyclonal to Mouse IgG at 1/5000 dilutionPredicted band size:36kdObserved band size:36kd)
Mouse GAPDH Antibody | anti-GAPDH antibody
Mouse Anti-GAPDH monoclonal Antibody
Applications
ELISA, Western Blot
Purity
>95%, Protein G Purified
Synonyms
GAPDH; Mouse Anti-GAPDH monoclonal Antibody; G3PD; GAPD; MGC88685; anti-GAPDH antibody
Host
Mouse
Isotype
IgG2b
Purity/Purification
>95%, Protein G Purified
Applicable Applications for anti-GAPDH antibody
ELISA (EIA), Western Blot (WB)
Storage Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate
Non-conjugated
Immunogen
Recombinant GAPDH protein
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
Western Blot (WB)
(All lanes: Mouse anti-GAPDH Monoclonal antibody at 1ug/mlLane 1:A549 whole cell lysateLane 2:HT-29 whole cell lysateLane 3:Jurkat whole cell lysateLane 4:U251 whole cell lysateLane 5:COLO205 whole cell lysateLane 6:MCF7 whole cell lysateLane 7:K562 whole cell lysateLane 8:Hela whole cell lysateLane 9:293T whole cell lysateSecondaryGoat polyclonal to Mouse IgG at 1/5000 dilutionPredicted band size:36kdObserved band size:36kd)
Western Blot (WB)
(All lanes: Mouse anti-GAPDH Monoclonal antibody at 1ug/mlLane 1:A549 whole cell lysateLane 2:HT-29 whole cell lysateLane 3:Jurkat whole cell lysateLane 4:U251 whole cell lysateLane 5:COLO205 whole cell lysateLane 6:MCF7 whole cell lysateLane 7:K562 whole cell lysateLane 8:Hela whole cell lysateLane 9:293T whole cell lysateSecondaryGoat polyclonal to Mouse IgG at 1/5000 dilutionPredicted band size:36kdObserved band size:36kd)
Related Product Information for anti-GAPDH antibody
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of 37kDa that is consisdered as a cellular enzyme involved in glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. At the molecular level, sequential steps lead to nuclear translocation of GAPDH during cell death as follows: first, a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) that is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS); second, the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it; third, the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. (?)-deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.
Product Categories/Family for anti-GAPDH antibody
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Product Notes
The GAPDH (Catalog #AAA1495524) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GAPDH can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the GAPDH for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAPDH, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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