Mouse GAPDH Monoclonal Antibody | anti-GAPDH antibody

GAPDH Monoclonal Antibody

Cat. Num. AAA7136002

• Reactivity: Human, Rat, Rabbit, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: GAPDH; Monoclonal Antibody; GAPDH Monoclonal Antibody; G3PD; GAPD; MGC88685; anti-GAPDH antibody

• Host: Mouse
• Reactivity: Human, Rat, Rabbit, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 10H4D6
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH7.4

• Applicable Applications for anti-GAPDH antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: WB: 1:5000-1:60000; IHC: 1:200-1:500; IF: 1:50-1:100; IP: 2ul-8ul
• Conjugation: Non-conjugated
• Immunogen: Recombinant Human GAPDH protein (2-335AA)
• Product Type: Monoclonal Antibody
• Immunogen Species: Human
• Target Names: GAPDH
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western Blot Positive WB detected in: U87 whole cell lysate, PC3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, MG-63 whole cell lysate, U251 whole cell lysate, SH-SY5Y whole cell lysate All lanes GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 5min )

Western Blot (WB)

(Western Blot Positive WB detected in: SP2/0 whole cell lysate, NIH/3T3 whole cell lysate, Raw264.7 whole cell lysate, Mouse heart tissue, Mouse kidney tissue All lanes GAPDH antibody at 1:5000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 36 KDa Observed band size: 36 KDa Exposure time: 5min )

Western Blot (WB)

(Western Blot: Positive WB detected in: Rat heart tissue, Rat liver tissue, Rat spleen tissue, Rat brain tissue, Rat skeletal tissue, Rat stomach tissue, Rat kidney tissueAll lanes GAPDH antibody at 1:5000Secondary: Goat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min)

Western Blot (WB)

(Western Blot: Positive WB detected in: Rabbit heart tissue, Rabbit kidney tissue, Rabbit skeletal muscle tissueAll lanes GAPDH antibody at 1:5000Secondary: Goat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min)

Western Blot (WB)

(Western Blot: Positive WB detected in: Hela whole cell lysate at 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug, 0.3125ug, 0.15625ug, 0.078ug All lanes:GAPDH antibody at 1:5000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min)

Western Blot (WB)

(Western Blot: Positive WB detected in: 15ug hela whole cell lysate GAPDH antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 36 KDaObserved band size: 36 KDaExposure time: 5min)

Immunohistochemistry (IHC)

(IHC image diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)

Immunohistochemistry (IHC)

(IHC image diluted at 1:500 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)

Immunohistochemistry (IHC)

(IHC image diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.)

Immunofluorescence (IF)

(Immunofluorescence staining of Hela cells with at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)

Immunofluorescence (IF)

(Immunofluorescence staining of HepG2 cells with at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).)

Immunoprecipitation (IP)

(Immunoprecipitating GAPDH in Hela whole cell lysate Lane 1: Mouse control IgG instead of MBS7136002 in Hela whole cell lysate. Lane 2: MBS7136002 (5ul) + Hela whole cell lysate (500ug) Lane 3: Hela whole cell lysate (10ug) For western blotting, the blot was detected with MBS7136002 at 1:5000, and a HRPconjugated Protein G antibody was used as the secondary antibody at 1:2000)

Related Product Information for anti-GAPDH antibody

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of 37kDa that is consisdered as a cellular enzyme involved in glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. At the molecular level, sequential steps lead to nuclear translocation of GAPDH during cell death as follows: first, a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) that is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS); second, the modified GAPDH becomes capable of binding with Siah1, an E3 ubiquitin ligase, and stabilizes it; third, the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1's nuclear localization signal, and degrades Siah1's substrates in the nucleus, which results in cytotoxicity. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease.-deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.

Product Categories/Family for anti-GAPDH antibody

Tag & Control Antibodies