Western Blot (WB)
(Western blot analysis of lysate from Y79 cell line, using PPEF2 Antibody (Center). MBS9205901 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Rabbit anti-Human PPEF2 Polyclonal Antibody | anti-PPEF2 antibody
PPEF2 Antibody (Center)
Western Blot (WB)
(Western blot analysis of lysate from Y79 cell line, using PPEF2 Antibody (Center). MBS9205901 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Western Blot (WB)
(Western blot analysis of lysate from Y79 cell line, using PPEF2 Antibody (Center). MBS9205901 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.)
Immunohistochemistry (IHC)
(PPEF2 Antibody (Center) (MBS9205901)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PPEF2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(PPEF2 Antibody (Center) (MBS9205901)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PPEF2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
phosphatase with EF-hand motif family. The protein contains a
protein phosphatase catalytic domain, and at least two EF-hand
calcium-binding motifs in its C terminus. Although its substrate(s)
is unknown, the encoded protein, which is expressed specifically in
photoreceptors and the pineal, has been suggested to play a role in
the visual system. This gene shares high sequence similarity with
the Drosophila retinal degeneration C (rdgC) gene. [provided by
RefSeq].
Ramulu, P., et al. Mol. Cell. Biol. 21(24):8605-8614(2001)
Sherman, P.M., et al. Proc. Natl. Acad. Sci. U.S.A. 94(21):11639-11644(1997)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein, which is expressed specifically in photoreceptors and the pineal, has been suggested to play a role in the visual system. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PPEF2: May play a role in phototransduction. May dephosphorylate photoactivated rhodopsin. May function as a calcium sensing regulator of ionic currents, energy production or synaptic transmission. Belongs to the PPP phosphatase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.3.16; Protein phosphatase, Ser/Thr (non-receptor); Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 4q21.1
Cellular Component: photoreceptor inner segment; photoreceptor outer segment; cytoplasm
Molecular Function: protein binding; iron ion binding; manganese ion binding; Hsp90 protein binding; mitogen-activated protein kinase kinase kinase binding; Hsp70 protein binding; calcium ion binding; protein serine/threonine phosphatase activity
Biological Process: visual perception; negative regulation of MAPKKK cascade; regulation of MAP kinase activity; regulation of JNK activity; detection of stimulus involved in sensory perception; protein amino acid dephosphorylation
Research Articles on PPEF2
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Product Notes
The PPEF2 ppef2 (Catalog #AAA9205901) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 304-333. The PPEF2 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPEF2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). WB~~1:1000. Researchers should empirically determine the suitability of the PPEF2 ppef2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPEF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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