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Western Blot (WB) (Western blotAll lanes: GALT antibody at 2ug/ml+mouse small intestine tissueSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 44,32 kDaObserved band size: 43 kDa)

Rabbit anti-Human, mouse Galactose-1-phosphate uridylyltransferase Polyclonal Antibody | anti-GALT antibody

Rabbit anti-human Galactose-1-phosphate uridylyltransferase polyclonal Antibody

Reactivity
Human, mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Caprylic Acid Ammonium Sulfate Precipitation purified
Synonyms
Galactose-1-phosphate uridylyltransferase; Polyclonal Antibody; Rabbit anti-human Galactose-1-phosphate uridylyltransferase polyclonal Antibody; UDP-glucose--hexose-1-phosphate uridylyltransferase GALT; anti-GALT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Caprylic Acid Ammonium Sulfate Precipitation purified
Form/Format
Liquid
Sequence Length
379
Applicable Applications for anti-GALT antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Recommended dilution: WB:1:500-2000
Immunogen
Recombinant human Galactose-1-phosphate uridylyltransferase protein
Conjugation
Non-conjugated
Storage Buffer
Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-162839
Preparation and Storage
Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.

Western Blot (WB)

(Western blotAll lanes: GALT antibody at 2ug/ml+mouse small intestine tissueSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 44,32 kDaObserved band size: 43 kDa)

Western Blot (WB) (Western blotAll lanes: GALT antibody at 2ug/ml+mouse small intestine tissueSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 44,32 kDaObserved band size: 43 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS7003933 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS7003933 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7003933 at dilution of 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7003933 at dilution of 1:100)

Western Blot (WB)

(Western BlotPositive WB detected in: Rat liver tissue,Mouse kidney tissueAll lanes: GALT antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 44,32 KDaObserved band size: 44 KDa)

Western Blot (WB) (Western BlotPositive WB detected in: Rat liver tissue,Mouse kidney tissueAll lanes: GALT antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 44,32 KDaObserved band size: 44 KDa)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells using MBS7003933 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells using MBS7003933 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-GALT antibody
Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined.

Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined.
Product Categories/Family for anti-GALT antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
31,107 Da
NCBI Official Full Name
galactose-1-phosphate uridylyltransferase isoform 1
NCBI Official Synonym Full Names
galactose-1-phosphate uridylyltransferase
NCBI Official Symbol
GALT
NCBI Protein Information
galactose-1-phosphate uridylyltransferase
UniProt Protein Name
Galactose-1-phosphate uridylyltransferase
Protein Family
UniProt Gene Name
GALT
UniProt Synonym Gene Names
Gal-1-P uridylyltransferase
UniProt Entry Name
GALT_HUMAN

NCBI Description

Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

GALT: Defects in GALT are the cause of galactosemia (GALCT). Galactosemia is an inherited disorder of galactose metabolism that causes jaundice, cataracts, and mental retardation. Belongs to the galactose-1-phosphate uridylyltransferase type 1 family.

Protein type: EC 2.7.7.12; Transferase; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: cytosol; Golgi apparatus

Molecular Function: protein binding; UDP-glucose:hexose-1-phosphate uridylyltransferase activity; zinc ion binding

Biological Process: carbohydrate metabolic process; galactose catabolic process; galactose metabolic process; UDP-glucose catabolic process

Disease: Galactosemia

Research Articles on GALT

Similar Products

Product Notes

The GALT galt (Catalog #AAA7003933) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human Galactose-1-phosphate uridylyltransferase polyclonal Antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Galactose-1-phosphate uridylyltransferase can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000. Researchers should empirically determine the suitability of the GALT galt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Galactose-1-phosphate uridylyltransferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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