Mouse anti-Human, Mouse Galectin-14 Monoclonal Antibody | anti-LGALS14 antibody
Galectin-14 (LGALS14 lectin, galactoside-binding, soluble, Charcot-Leyden crystal protein 2, CLC2, MGC22235, Placental protein 13-like, PPL13)
Gene Names
LGALS14; CLC2; PPL13; MGC22235; LGALS14
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein G immunoaffinity chromatography.
Synonyms
Galectin-14; Monoclonal Antibody; Galectin-14 (LGALS14 lectin; galactoside-binding; soluble; Charcot-Leyden crystal protein 2; CLC2; MGC22235; Placental protein 13-like; PPL13); Anti -Galectin-14 (LGALS14 lectin; anti-LGALS14 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
9H126
Specificity
Detects rhGalectin-14 in direct ELISAs and Western blots. In direct ELISAs, Shows no cross-reactivity with rhGalectin-1, 2, 3, 4, 7, 8, 10, or rmGalectin-9.
Purity/Purification
Affinity Purified
Purified by Protein G immunoaffinity chromatography.
Purified by Protein G immunoaffinity chromatography.
Form/Format
Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.2ml of PBS is used, the antibody concentration will be 500ug/ml.
Applicable Applications for anti-LGALS14 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 2ug/ml with the appropriate secondary reagents to detect human Galectin-14 in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from primary human eosinophils in non-reducing sample buffer, resolved by SDS-PAGE 10ug total protein/lane), and transferred to an Blotting Buffer Blocking Solution Antibody Solution 25mM Tris, pH 7.4 5% nonfat dry milk 5% nonfat dry milk 0.15M NaCl in Blotting Buffer in Blotting Buffer 0.05% Tween 20.
Direct ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect human Galectin-14. The detection limit for rhGalectin-14 is approximately 3ng/well.
Dilution: Western Blot: 2ug/ml with the appropriate secondary reagents to detect human Galectin-14 in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from primary human eosinophils in non-reducing sample buffer, resolved by SDS-PAGE 10ug total protein/lane), and transferred to an Blotting Buffer Blocking Solution Antibody Solution 25mM Tris, pH 7.4 5% nonfat dry milk 5% nonfat dry milk 0.15M NaCl in Blotting Buffer in Blotting Buffer 0.05% Tween 20.
Direct ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect human Galectin-14. The detection limit for rhGalectin-14 is approximately 3ng/well.
Immunogen
Purified, E. coli-derived, recombinant human Galectin-14 (rhGalectin-14; aa 1-139; Accession# Q8TCE9).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-LGALS14 antibody
Galectin-14, also known as placental protein 13-like (PPL13), belongs the galectin family of carbohydrate binding proteins. It is expressed intracellularly in placenta and eosinophils and is released by eosinophils following allergen stimulation. Galectin-14 may be involved in the development of allergic inflammation.
Product Categories/Family for anti-LGALS14 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16,094 Da
NCBI Official Full Name
galectin-14 transcription variant 1
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 14
NCBI Official Symbol
LGALS14
NCBI Official Synonym Symbols
CLC2; PPL13; MGC22235; LGALS14
NCBI Protein Information
placental protein 13-like; gal-14; galectin-14; Charcot-Leyden crystal protein 2; placental protein 13-like protein
UniProt Protein Name
Placental protein 13-like
Protein Family
UniProt Gene Name
LGALS14
UniProt Synonym Gene Names
PPL13
UniProt Entry Name
PPL13_HUMAN
NCBI Description
This gene is predominantly expressed in placenta. The encoded protein belongs to the galectin (galaptin/S-lectin) family. The members of galectin family contain one or two carbohydrate recognition domains, which can bind beta-galactoside. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]
Uniprot Description
LGALS14: 2 isoforms of the human protein are produced by alternative splicing
Chromosomal Location of Human Ortholog: 19q13.2
Cellular Component: nucleus
Molecular Function: carbohydrate binding
Biological Process: apoptosis
Research Articles on LGALS14
1. CIC-2 siRNA could inhibit the cell proliferation of a human glioma cell line U-87.
2. Observational study of gene-disease association. (HuGE Navigator)
3. cloning and expression in placenta - base sequence and amino acid sequences are given
2. Observational study of gene-disease association. (HuGE Navigator)
3. cloning and expression in placenta - base sequence and amino acid sequences are given
Similar Products
Product Notes
The LGALS14 lgals14 (Catalog #AAA605811) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Galectin-14 (LGALS14 lectin, galactoside-binding, soluble, Charcot-Leyden crystal protein 2, CLC2, MGC22235, Placental protein 13-like, PPL13) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Galectin-14 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 2ug/ml with the appropriate secondary reagents to detect human Galectin-14 in cell or tissue extracts under non-reducing conditions only. Lysates were prepared from primary human eosinophils in non-reducing sample buffer, resolved by SDS-PAGE 10ug total protein/lane), and transferred to an Blotting Buffer Blocking Solution Antibody Solution 25mM Tris, pH 7.4 5% nonfat dry milk 5% nonfat dry milk 0.15M NaCl in Blotting Buffer in Blotting Buffer 0.05% Tween 20. Direct ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect human Galectin-14. The detection limit for rhGalectin-14 is approximately 3ng/well. Researchers should empirically determine the suitability of the LGALS14 lgals14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Galectin-14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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