Mouse anti-Yeast GAL-4 Activation Domain Monoclonal Antibody | anti-GAL4 antibody
GAL-4 Activation Domain
Gene Names
GAL4; GAL81
Reactivity
Yeast
Applications
Western Blot, Immunoprecipitation
Purity
Protein A/G Chromatography
Synonyms
GAL-4 Activation Domain; Monoclonal Antibody; anti-GAL4 antibody
Host
Mouse
Reactivity
Yeast
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5C8-5
Purity/Purification
Protein A/G Chromatography
Form/Format
Unconjugated
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Sequence Length
881
Applicable Applications for anti-GAL4 antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Antibody can be used for Western Blotting (5-10 ug/mL). Recognizes GAL4 derivatives containing the C-terminal activation domain. Optimal concentration should be eevaluated by serial dilutions.
Immunogen
Hybridoma produced by the fusion of splenocytes from mice immunized with full length GAL4 expressed in E. Coli and mouse myeloma cells.
Functional Analysis
Western Blotting
Preparation and Storage
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
Shipping conditions: Ship at ambient temperature, freeze upon arrival.
Shipping conditions: Ship at ambient temperature, freeze upon arrival.
Related Product Information for anti-GAL4 antibody
GAL4 is a transcriptional activator which activates genes necessary for galactose catabolism. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a zinc finger sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription.
Product Categories/Family for anti-GAL4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
99,403 Da
NCBI Official Full Name
Gal4p
NCBI Official Symbol
GAL4
NCBI Official Synonym Symbols
GAL81
NCBI Protein Information
Gal4p
UniProt Protein Name
Regulatory protein GAL4
Protein Family
UniProt Gene Name
GAL4
UniProt Entry Name
GAL4_YEAST
Uniprot Description
Function: This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.
Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Ref.5 Ref.6
Subcellular location: Nucleus.
Domain: the 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Ref.8
Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4.
Miscellaneous: Present with 166 molecules/cell in log phase SD medium.
Sequence similarities: Contains 1 Zn(2)-C6 fungal-type DNA-binding domain.
Research Articles on GAL4
Similar Products
Product Notes
The GAL4 gal4 (Catalog #AAA395014) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GAL-4 Activation Domain reacts with Yeast and may cross-react with other species as described in the data sheet. AAA Biotech's GAL-4 Activation Domain can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Antibody can be used for Western Blotting (5-10 ug/mL). Recognizes GAL4 derivatives containing the C-terminal activation domain. Optimal concentration should be eevaluated by serial dilutions. Researchers should empirically determine the suitability of the GAL4 gal4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAL-4 Activation Domain, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
