Western Blot/Blotting
(Western blot of His tagged protein (vector pET 30C) using anti-6x His antibody. (1) Whole cellular extract of BL21 (DE3) pJT4pTT9 bacteria carrying gene encoding for His-Rel E)
Mouse 6x-His Tag Monoclonal Antibody | anti-6x-His Tag antibody
6x-His Tag
Applications
Western Blot
Purity
Protein A/G Chromatography
Synonyms
6x-His Tag; Monoclonal Antibody; Anti-6x-Histidine Tag Antibody; anti-6x-His Tag antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
2C5.1
Purity/Purification
Protein A/G Chromatography
Form/Format
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Applicable Applications for anti-6x-His Tag antibody
Western Blot (WB)
Immunogen
Hybridoma produced by the fusion of splenocytes from mice immunized with His-His-His-His-His-His synthetic peptide and mouse myeloma cells.
Preparation and Storage
Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles
Western Blot/Blotting
(Western blot of His tagged protein (vector pET 30C) using anti-6x His antibody. (1) Whole cellular extract of BL21 (DE3) pJT4pTT9 bacteria carrying gene encoding for His-Rel E)
Western Blot/Blotting
(Western blot of His tagged protein (vector pET 30C) using anti-6x His antibody. (1) Whole cellular extract of BL21 (DE3) pJT4pTT9 bacteria carrying gene encoding for His-Rel E)
Related Product Information for anti-6x-His Tag antibody
6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems. This antibody has been found to be effective in detecting proteins expressed using the following vector systems, pET 28, 30 and 32 as well as Invitrogen's pIZ and is specific for 6x-His tags only.
Product Categories/Family for anti-6x-His Tag antibody
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Product Notes
The 6x-His Tag (Catalog #AAA395186) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 6x-His Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the 6x-His Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "6x-His Tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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