Western Blot (WB)
(Figure 1: Western blot analysis using GAB1 mAb against human GAB1 (AA: 661-724) recombinant protein. (Expected MW is 32.4 kDa))
Mouse anti-Human, Mouse GAB1 Monoclonal Antibody | anti-GAB1 antibody
GAB1 Antibody
Gene Names
GAB1; DFNB26
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA, Flow Cytometry
Purity
Peptide Affinity-Chromatography
Synonyms
GAB1; Monoclonal Antibody; GAB1 Antibody; Gab 1; GAB1_HUMAN; GRB 2 associated binder 1; GRB 2 associated binding protein 1; GRB2 associated binding protein 1 isoform a; GRB2 associated binding protein 1 isoform b; GRB2-associated binder 1; GRB2-associated-binding protein 1; Growth factor receptor bound protein 2-associated protein 1; anti-GAB1 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
GAB1 antibody detects endogenous levels of total GAB1
Purity/Purification
Peptide Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
694
Applicable Applications for anti-GAB1 antibody
Western Blot (WB), Immunohistochemisty (IHC), ELISA (EIA), Flow Cytometry (FC)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
FC: 1:200-1:400
The optimal dilution should be determined by the end users.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
WB: 1:500-1:2000
IHC: 1:200-1:1000
FC: 1:200-1:400
The optimal dilution should be determined by the end users.
IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
Purified recombinant fragment of human GAB1 expressed in E. Coli
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using GAB1 mAb against human GAB1 (AA: 661-724) recombinant protein. (Expected MW is 32.4 kDa))
Western Blot (WB)
(Figure 1: Western blot analysis using GAB1 mAb against human GAB1 (AA: 661-724) recombinant protein. (Expected MW is 32.4 kDa))
Related Product Information for anti-GAB1 antibody
Description: Grb2-associated binding protein 1 is a member of the IRS-1-like multisubstrate docking protein family. Contains an N-terminal pleckstrin homology (PH) domain and several proline-rich SH3 domain-binding sequences. Mediates branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
76.6 kDa
NCBI Official Full Name
GRB2-associated-binding protein 1 isoform b
NCBI Official Synonym Full Names
GRB2 associated binding protein 1
NCBI Official Symbol
GAB1
NCBI Official Synonym Symbols
DFNB26
NCBI Protein Information
GRB2-associated-binding protein 1
UniProt Protein Name
GRB2-associated-binding protein 1
Protein Family
UniProt Gene Name
GAB1
NCBI Description
The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
Uniprot Description
Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Involved in the MET/HGF-signaling pathway (PubMed:29408807).
Research Articles on GAB1
Similar Products
Product Notes
The GAB1 gab1 (Catalog #AAA9602566) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GAB1 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GAB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), ELISA (EIA), Flow Cytometry (FC). ELISA: 1:10000 WB: 1:500-1:2000 IHC: 1:200-1:1000 FC: 1:200-1:400 The optimal dilution should be determined by the end users. IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. . Researchers should empirically determine the suitability of the GAB1 gab1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GAB1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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