Mouse anti-Human Gab1 Monoclonal Antibody | anti-Gab1 antibody

Gab1 (GAB1, GRB2-associated-binding protein 1, GRB2-associated binder 1, Growth factor receptor bound protein 2-associated protein 1) (AP)

Cat. Num. AAA6120829

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Gab1; Monoclonal Antibody; Gab1 (GAB1; GRB2-associated-binding protein 1; GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1) (AP); GAB1; Growth factor receptor bound protein 2-associated protein 1; anti-Gab1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1AT1979
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-Gab1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1,000; WB: 1:200-1000; Applications are based on unconjugated antibody.
• Immunogen: Gab1 antibody is generated from mice immunized with a GST fusion protein encoding full length human GAB1.
• Conjugate: AP
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C with the addition of glycerol. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive.

Immunoprecipitation (IP)

(Wild-type (+/+) and Gab1-/- (-/-) cells were heat shocked (HS) at 42 degree C for 1 h or irradiated with UV-B light (400 J/m2) and then incubated at 37? for 1 h. Cell lysates were prepared and subjected to immunoprecipitation (IP) with an anti-JNK2 antibody. The immunoprecipitates were resolved by SDS- PAGE and immunoblotted (IB) with anti-Gab1 antibody. Data courtesy of Dr. GS Feng at The Burnham Institute.)

Testing Data

(Rescue of the JNK pathway by expression of wild-type Gab1 in Gab1-/- cells. Gab1-/- cells were transiently transfected with 1, 2, or 4 of the expression construct for human Gab1 cDNA, using the GenePORTER 2 transfection reagent (Gene Therapy Systems Inc.). After incubation, the cells were irradiated with UV-B light at 400 J/m2 or left untreated. Exogenous expression of Gab1 in transfected cells was confirmed by anti-Gab1 immunoblot analysis with wild-type cells as positive control. +/+, wild-type cells; -/-, Gab1-/- cells. (Mol. Cell. Biol. 2004 Feb 15;24 (4):1531-1539))

Related Product Information for anti-Gab1 antibody

The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-Gab1 antibody

Antibodies; Transcription Factors

References

An approach based on a genome-wide association study reveals candidate loci for narcolepsy. Shimada M, et al. Hum Genet, 2010 Oct. PMID 20677014. Variation at the NFATC2 Locus Increases the Risk of Thiazolinedinedione-Induced Edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) Study. Bailey SD, et al. Diabetes Care, 2010 Jul 13. PMID 20628086. Grb2-associated binder 1 (Gab1) genetic polymorphism, Helicobacter pylori infection, and chronic atrophic gastritis among older adults from Germany. Gao L, et al. Mol Carcinog, 2010 Oct. PMID 20602450. New genetic associations detected in a host response study to hepatitis B vaccine. Davila S, et al. Genes Immun, 2010 Apr. PMID 20237496. Assembly of the Sos1-Grb2-Gab1 ternary signaling complex is under allosteric control. McDonald CB, et al. Arch Biochem Biophys, 2010 Feb 15. PMID 20005866.

NCBI and Uniprot Product Information

• NCBI GI #: 46370073
• NCBI GeneID: 2549
• NCBI Accession #: NP_002030.2
• NCBI GenBank Nucleotide #: NM_002039.3
• UniProt Accession #: Q13480; Q4W5G2; Q6P1W2; A8K152
• Molecular Weight: 76,616 Da
• NCBI Official Full Name: GRB2-associated-binding protein 1 isoform b
• NCBI Official Synonym Full Names: GRB2-associated binding protein 1
• NCBI Official Symbol: GAB1
• NCBI Protein Information: GRB2-associated-binding protein 1; GRB2-associated binder 1; growth factor receptor bound protein 2-associated protein 1
• UniProt Protein Name: GRB2-associated-binding protein 1
• Protein Family: GPI transamidase component
• UniProt Gene Name: GAB1
• UniProt Entry Name: GAB1_HUMAN