G6PD active protein

G6PD, 1-515aa, Human, His tag, Baculovirus (Bioactivity Validated)

Cat. Num. AAA204787

• Gene Names: G6PD; G6PD1
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: G6PD; 1-515aa; Human; His tag; Baculovirus (Bioactivity Validated); glucose-6-phosphate 1-dehydrogenase; G6PD1; G6PD active protein

• Host: Baculovirus
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.1mM PMSF, 2mM EDTA, 2mM DTT, 200mM NaCl
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MAEQVALSRT QVCGILREEL FQGDAFHQSD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL LPENTFIVGY ARSRLTVADI RKQSEPFFKA TPEEKLKLED FFARNSYVAG QYDDAASYQR LNSHMNALHL GSQANRLFYL ALPPTVYEAV TKNIHESCMS QIGWNRIIVE KPFGRDLQSS DRLSNHISSL FREDQIYRID HYLGKEMVQN LMVLRFANRI FGPIWNRDNI ACVILTFKEP FGTEGRGGYF DEFGIIRDVM QNHLLQMLCL VAMEKPASTN SDDVRDEKVK VLKCISEVQA NNVVLGQYVG NPDGEGEATK GYLDDPTVPR GSTTATFAAV VLYVENERWD GVPFILRCGK ALNERKAEVR LQFHDVAGDI FHQQCKRNEL VIRVQPNEAV YTKMMTKKPG MFFNPEESEL DLTYGNRYKN VKLPDAYERL ILDVFCGSQM HFVRSDELRE AWRIFTPLLH QIELEKPKPI PYIYGSRGPT EADELMKRVG FQYEGTYKWV NPHKL
• Sequence Length: 515

• Applicable Applications for G6PD active protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Biological Activity: Specific activity is > 45 units/mg, obtained by measuring the increase of beta-NADPH in absorbance at 340 nm resulting from the reduction of beta-NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C.
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for G6PD active protein

Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant human G6PD protein, fused to His-tag at N-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.

Product Categories/Family for G6PD active protein

Enzymes & Proteases

References

Huang Y., et al. (2008) Mol Genet Metab. 93 (1):44-53.; Zimny A., et al. (2003) Pol Arch Med Wewn. 110 (5):1327-33

NCBI and Uniprot Product Information

• NCBI GI #: 108773793
• NCBI GeneID: 2539
• NCBI Accession #: NP_001035810.1
• NCBI GenBank Nucleotide #: NM_001042351.2
• UniProt Accession #: P11413; Q16000; Q16765; Q8IU70; Q8IU88; Q8IUA6; Q96PQ2; D3DWX9
• Molecular Weight: 61.4kDa (535aa)
• NCBI Official Full Name: glucose-6-phosphate 1-dehydrogenase isoform b
• NCBI Official Synonym Full Names: glucose-6-phosphate dehydrogenase
• NCBI Official Symbol: G6PD
• NCBI Official Synonym Symbols: G6PD1
• NCBI Protein Information: glucose-6-phosphate 1-dehydrogenase
• UniProt Protein Name: Glucose-6-phosphate 1-dehydrogenase
• UniProt Gene Name: G6PD
• UniProt Synonym Gene Names: G6PD

NCBI Description

This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.