Mouse Fructose-1,6-Bisphosphatase 2 Monoclonal Antibody | anti-FBP2 antibody

Mouse Anti Human Fructose-1,6-Bisphosphatase 2

Cat. Num. AAA140430

• Applications: ELISA, Western Blot
• Purity: FBP2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Synonyms: Fructose-1; 6-Bisphosphatase 2; Monoclonal Antibody; Mouse Anti Human Fructose-1; FBP2 Antibody; Mouse Anti Human; 6-bisphosphatase isozyme 2; FBPase 2; D-fructose-1; 6-bisphosphate 1-phosphohydrolase 2; FBP2; anti-FBP2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 heavy chain
• Clone Number: PAT1E11AT
• Purity/Purification: FBP2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Form/Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.; Sterile Filtered colorless solution.
• Sequence Length: 339

• Applicable Applications for anti-FBP2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: FBP2 antibody has been tested by ELISA, Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1:5000. Recommended starting dilution is 1:1000.
• Immunogen: Anti-human FBP2 mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CMBL 1-339 amino acids purified from E Coli.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-FBP2 antibody

Introduction: Fructose-1,6-bisphosphatase isozyme 2 (FBP2) is a part of the FBPase class 1 family. FBP2 is a gluconeogenesis regulatory enzyme Which catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate.

Product Categories/Family for anti-FBP2 antibody

Monoclonal Antibodies; Anti-Human Enzymes; Monoclonal Antibodies; Anti Human Enzyme

NCBI and Uniprot Product Information

• NCBI GI #: 109731177
• NCBI GeneID: 8789
• Molecular Weight: 36,743 Da
• NCBI Official Full Name: Fructose-1,6-bisphosphatase 2
• NCBI Official Synonym Full Names: fructose-1,6-bisphosphatase 2
• NCBI Official Symbol: FBP2
• NCBI Protein Information: fructose-1,6-bisphosphatase isozyme 2; D-fructose-1,6-bisphosphate 1-phosphohydrolase 2; FBPase 2; hexosediphosphatase; muscle FBPase; muscle fructose-bisphosphatase
• UniProt Protein Name: Fructose-1,6-bisphosphatase isozyme 2
• Protein Family: Fat body protein
• UniProt Gene Name: FBP2
• UniProt Synonym Gene Names: FBPase 2
• UniProt Entry Name: F16P2_HUMAN

NCBI Description

This gene encodes a gluconeogenesis regulatory enzyme which catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. [provided by RefSeq, Jul 2008]

Uniprot Description

FBPase 2: a key enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, a precursor to glucose 6-phosphate. A regulator of glucose synthesis from non-carbohydrates. Two paralogs of this enzyme exists in humans: FBP1 is expressed in the liver and gluconeogenic tissues, and FBP2 in striated muscle. FBP2 is only isozyme expressed in striated muscle and it is widely expressed in cells that are nongluconeogenic. Both forms are inhibited allosterically by AMP, NAD and Ca2+. The muscle form is about 100-fold more sensitive to AMP and NAD, and about 1000-fold more sensitive to inhibition by Ca2+, the major activator of glycolysis in striated muscle. Forms an active complex with aldolase that binds to the sarcomeric Z-line, which is disrupted by elevated calcium levels, blocking glycogen synthesis during exercise. Forms homotetramers that are stabilized in the active state by divalent cations (Mg2+, Mn2+ , Co+2, or Zn2+). Its interaction with aldolase appears to desensitizes it to inhibition by AMP and, partially, by Ca2+. Up to 50% of lactate generated in striated muscle is converted to glycogen.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Phosphatase (non-protein); Carbohydrate Metabolism - pentose phosphate pathway; EC 3.1.3.11

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: Z disc; cytosol; cell junction; nucleus

Molecular Function: metal ion binding; fructose-bisphosphatase activity

Biological Process: dephosphorylation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; fructose metabolic process; gluconeogenesis