Mouse FOXP3 (Forkhead Box Protein P3) / Scurfin Monoclonal Antibody | anti-FOXP3 antibody

FOXP3 (Forkhead Box Protein P3) / Scurfin Mouse Monoclonal Antibody

Cat. Num. AAA438165

• Gene Names: FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER
• Reactivity: Human, Monkey, Mouse
• Applications: Flow Cytometry, Immunohistochemistry, Western Blot
• Synonyms: FOXP3 (Forkhead Box Protein P3) / Scurfin; Monoclonal Antibody; FOXP3 (Forkhead Box Protein P3) / Scurfin Mouse Monoclonal Antibody; JM2; AIID; DIETER; IPEX; MGC141961; MGC141963; PIDX; XPID; FOXP3delta7; forkhead box protein P3; immune dysregulation; polyendocrinopathy; enteropathy; X-linked; immunodeficiency; scurfin; anti-FOXP3 antibody

• Host: Mouse
• Reactivity: Human, Monkey, Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: SPM579
• Specificity: Recognizes a protein of 47-55kDa, which is identified as FOXP3. Its precise epitope is not known, but it has been mapped to the N-terminal portion of the protein. The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. During early development, FOXP1 and FOXP2 are expressed abundantly in the lung, with lower levels of expression in neural, intestinal and cardiovascular tissues, where they act as transcription repressors. FOXP1 is widely expressed in adult tissues, while neoplastic cells often exhibit a dramatic change in expression level or localization of FOXP1. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 396

• Applicable Applications for anti-FOXP3 antibody: Flow Cytometry (FC), Immunohistochemistry (IHC) (Formalin-Fixed), Western Blot (WB)
• Application Notes: Flow Cytometry (1-2ug/million cells); Western Blot (WB) ( 1-2ug/mL); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
• Cellular Localization: Predominantly nuclear, some cytoplasmic
• Immunogen: Full-length human FOXP3 protein
• Positive Control: Tonsil, lymph node or Breast carcinoma
• Human Chromosome Location: XP1123
• Preparation and Storage: Antibody without azide , store at -20°C to -80°C. Non hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with FOXP3 Monoclonal Antibody (SPM579).)

References

Schubert, L.A., et al. 2001. Scurfin (FOXP3) acts as a repressor of transcription and regulates T cell activation. J. Biol. Chem. 276: 37672-37679

NCBI and Uniprot Product Information

• NCBI GI #: 167466190
• NCBI GeneID: 50943
• NCBI Accession #: NP_001107849.1
• NCBI GenBank Nucleotide #: NM_001114377.1
• UniProt Accession #: Q9BZS1; O60827; Q14DD8; Q4ZH51; A5HJT1; B7ZLG0; B9UN80
• Molecular Weight: 47-55kDa
• NCBI Official Full Name: forkhead box protein P3 isoform b
• NCBI Official Synonym Full Names: forkhead box P3
• NCBI Official Symbol: FOXP3
• NCBI Official Synonym Symbols: JM2; AIID; IPEX; PIDX; XPID; DIETER
• NCBI Protein Information: forkhead box protein P3
• UniProt Protein Name: Forkhead box protein P3
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXP3
• UniProt Synonym Gene Names: IPEX
• UniProt Entry Name: FOXP3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXP3: Probable transcription factor. Plays a critical role in the control of immune response. Interacts with IKZF3. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Transcription factor; DNA-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: cytoplasm; nucleus

Molecular Function: histone acetyltransferase binding; histone deacetylase binding; NFAT protein binding; protein binding; protein homodimerization activity; sequence-specific DNA binding; transcription factor activity

Biological Process: CD4-positive, CD25-positive, alpha-beta regulatory T cell lineage commitment; inhibition of CREB transcription factor; inhibition of NF-kappaB transcription factor; negative regulation of cell proliferation; negative regulation of cytokine biosynthetic process; negative regulation of cytokine secretion; negative regulation of histone deacetylation; negative regulation of immune response; negative regulation of interferon-gamma production; negative regulation of interleukin-10 production; negative regulation of interleukin-17 production; negative regulation of interleukin-2 biosynthetic process; negative regulation of interleukin-2 production; negative regulation of interleukin-4 production; negative regulation of T cell cytokine production; negative regulation of T cell proliferation; negative regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of T cell anergy; response to virus; T cell activation

Disease: Diabetes Mellitus, Insulin-dependent; Immunodysregulation, Polyendocrinopathy, And Enteropathy, X-linked