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SDS-Page (SDS-PAGE Analysis Purified FOXL1 Mouse Monoclonal Antibody (PCRP-FOXL1-1F8). Confirmation of Purity and Integrity of Antibody. )

Mouse FOXL1/Forkhead Box Protein L1 Monoclonal Antibody | anti-FOXL1 antibody

FOXL1/Forkhead Box Protein L1 (Transcription Factor) Mouse Monoclonal Antibody [Clone PCRP-FOXL1-1F8]

Gene Names
FOXI1; HFH3; FKH10; HFH-3; FKHL10; FREAC6; FREAC-6
Reactivity
Human
Predicted to react in Rat.
Applications
Immunoprecipitation, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
Synonyms
FOXL1/Forkhead Box Protein L1; Monoclonal Antibody; FOXL1/Forkhead Box Protein L1 (Transcription Factor) Mouse Monoclonal Antibody [Clone PCRP-FOXL1-1F8]; FOXL1/Forkhead Box Protein L1 (Transcription Factor); FKHL10; FREAC6; JFJ-3; FKH6; FKH11; Forkhead box protein L1; Forkhead related transcription factor; FREAC7; anti-FOXL1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Predicted to react in Rat.
Clonality
Monoclonal
Isotype
Mouse/IgG1
Clone Number
PCRP-FOXL1-1F8
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0. 05% BSA & 0. 05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
Purified Ab with BSA and Azide at 200ug/ml
OR
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml (varies by lot)
Applicable Applications for anti-FOXL1 antibody
Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Formalin (IHC-F)
Application Notes
IP: 1-2ug per 100-500ug of total protein)
FC/FACS: 1-2ug/million cells
IF: 1-2ug/ml
WB: 1-2ug/ml
IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT
(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes)
Immunogen
Recombinant fragment (around aa104-258) of human FOXL1 protein (exact sequence is proprietary)
Cellular Localization
Nucleus
Positive Control
HeLa or U87 cells. Ubiquitous tissue expression.
Hu-chromosome Location
16q24.1
Unigene
87236
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C
Antibody without azide: store at -20 to -80 degree C
Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified FOXL1 Mouse Monoclonal Antibody (PCRP-FOXL1-1F8). Confirmation of Purity and Integrity of Antibody. )

SDS-Page (SDS-PAGE Analysis Purified FOXL1 Mouse Monoclonal Antibody (PCRP-FOXL1-1F8). Confirmation of Purity and Integrity of Antibody. )
Related Product Information for anti-FOXL1 antibody
FOXL1 is a 337 amino acid protein encoded by the mouse gene FOXL1. FOXL1 belongs to the forkhead family and contains one forkhead DNA-binding domain. The HNF3/forkhead family includes a large number of transcription factors that share a structurally related DNA binding domain. forkhead factors are known to play important roles both during development and in adults. FOXL1 is a winged helix transcriptional regulator expressed in the mesenchymal layer of developing and mature gastrointestinal tract. FOXL1-deficient mice exhibit various defects not only in the epithelial layer of the gastrointestinal tract but also in gut-associated lymphoid tissues. In the small intestine of FOXL1-deficient mice, the formation of Peyer's patches is affected, particularly in the caudal region. FOXL1 is a mesenchymal modifier of the Adenomatous Polyposis Coli (APC) gene products and plays a key role in gastrointestinal tumorigenesis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,814 Da
NCBI Official Full Name
forkhead box protein I1 isoform a
NCBI Official Synonym Full Names
forkhead box I1
NCBI Official Symbol
FOXI1
NCBI Official Synonym Symbols
HFH3; FKH10; HFH-3; FKHL10; FREAC6; FREAC-6
NCBI Protein Information
forkhead box protein I1; forkhead-like 10; HNF-3/fork-head homolog 3; HNF-3/fork-head homolog-3; forkhead-related activator 6; forkhead-related protein FKHL10; forkhead-related transcription factor 6; hepatocyte nuclear factor 3 forkhead homolog 3
UniProt Protein Name
Forkhead box protein I1
Protein Family
UniProt Gene Name
FOXI1
UniProt Synonym Gene Names
FKHL10; FREAC6; FREAC-6; HFH-3; HNF-3/fork-head homolog 3
UniProt Entry Name
FOXI1_HUMAN

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Product Notes

The FOXL1 foxi1 (Catalog #AAA4381894) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The FOXL1/Forkhead Box Protein L1 (Transcription Factor) Mouse Monoclonal Antibody [Clone PCRP-FOXL1-1F8] reacts with Human Predicted to react in Rat. and may cross-react with other species as described in the data sheet. AAA Biotech's FOXL1/Forkhead Box Protein L1 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry-Formalin (IHC-F). IP: 1-2ug per 100-500ug of total protein) FC/FACS: 1-2ug/million cells IF: 1-2ug/ml WB: 1-2ug/ml IHC (Formalin-fixed): 1-2ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH9.0, for 45 minutes at 95 degree C followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the FOXL1 foxi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXL1/Forkhead Box Protein L1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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