Mouse FKBP4 / FKBP52 Monoclonal Antibody | anti-FKBP4 antibody

Mouse Monoclonal [clone Hi52C] (IgG) to Human FKBP4 / FKBP52

Cat. Num. AAA245560

• Gene Names: FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase
• Reactivity: Dog, Hamster, Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, Immunoprecipitation
• Purity: Protein G Purified
• Synonyms: FKBP4 / FKBP52; Monoclonal Antibody; Mouse Monoclonal [clone Hi52C] (IgG) to Human FKBP4 / FKBP52; Anti-FKBP4 / FKBP52 Antibody (clone Hi52C) IHC-plus; FKBP4; 59 kDa immunophilin; 51 kDa FK506-binding protein; 52 kDa FK506-binding protein; 52 kDa FKBP; FKBP-52; FKBP51; HBI; Immunophilin FKBP52; FK506 binding protein 4; 59kDa; FK506-binding protein 4; HSP56; p52; p59; Rotamase; p59 protein; PPIase; Human FKBP4; FKBP52; anti-FKBP4 antibody

• Host: Mouse
• Reactivity: Dog, Hamster, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: Hi52C
• Specificity: Detects an ~52 kD protein representing FKBP52 in HeLa cell lysate. Also detects FKBP52 in whole tissue extracts from rat kidney and rat and mouse testes. Heavy chain migrates close to FKBP52 on SDS-PAGE.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 50% glycerol, 0.09% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 459

• Applicable Applications for anti-FKBP4 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: IF, IHC-P (10 ug/ml), IP, WB (1:2000); Usage: Immunoprecipitation: 5 ug with 10-20 ul Protein A beads.
• Target Species: Human
• Immunogen Description: Synthetic peptide corresponding to the residues of human FKBP52.
• Immunogen Type: Synthetic peptide
• Immunogen: FKBP4 / FKBP52 antibody was raised against synthetic peptide corresponding to the residues of human FKBP52.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-FKBP52 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Prostate tissue was histologically stained with antibodies specific for FKBP52. The strongest staining for FKBP52 is in ductal epithelial cells.)

Immunofluorescence (IF)

(FKBP52 (Hi52C), MCF-7 cells.)

Western Blot (WB)

(FKBP52 (Hi52C), Cell lines.)

Related Product Information for anti-FKBP4 antibody

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506.

NCBI and Uniprot Product Information

• NCBI GI #: 4503729
• NCBI GeneID: 2288
• NCBI Accession #: NP_002005.1
• NCBI GenBank Nucleotide #: NM_002014.3
• UniProt Accession #: Q02790; Q9UCP1; Q9UCV7; D3DUQ1
• Molecular Weight: 51,805 Da
• NCBI Official Full Name: peptidyl-prolyl cis-trans isomerase FKBP4
• NCBI Official Synonym Full Names: FK506 binding protein 4, 59kDa
• NCBI Official Symbol: FKBP4
• NCBI Official Synonym Symbols: HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase
• NCBI Protein Information: peptidyl-prolyl cis-trans isomerase FKBP4; rotamase; HSP binding immunophilin; 51 kDa FK506-binding protein; FK506-binding protein 4 (59kD); T-cell FK506-binding protein, 59kD; peptidylprolyl cis-trans isomerase
• UniProt Protein Name: Peptidyl-prolyl cis-trans isomerase FKBP4
• Protein Family: Peptidyl-prolyl cis-trans isomerase
• UniProt Gene Name: FKBP4
• UniProt Synonym Gene Names: FKBP52; PPIase FKBP4; FKBP51; 52 kDa FKBP; FKBP-52; p59; FKBP-4; HBI
• UniProt Entry Name: FKBP4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It has high structural and functional similarity to FK506-binding protein 1A (FKBP1A), but unlike FKBP1A, this protein does not have immunosuppressant activity when complexed with FK506. It interacts with interferon regulatory factor-4 and plays an important role in immunoregulatory gene expression in B and T lymphocytes. This encoded protein is known to associate with phytanoyl-CoA alpha-hydroxylase. It can also associate with two heat shock proteins (hsp90 and hsp70) and thus may play a role in the intracellular trafficking of hetero-oligomeric forms of the steroid hormone receptors. This protein correlates strongly with adeno-associated virus type 2 vectors (AAV) resulting in a significant increase in AAV-mediated transgene expression in human cell lines. Thus this encoded protein is thought to have important implications for the optimal use of AAV vectors in human gene therapy. The human genome contains several non-transcribed pseudogenes similar to this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

FKBP4: Immunophilin protein with PPIase and co-chaperone activities. Component of unligated steroid receptors heterocomplexes through interaction with heat-shock protein 90 (HSP90). May play a role in the intracellular trafficking of heterooligomeric forms of steroid hormone receptors between cytoplasm and nuclear compartments. The isomerase activity controls neuronal growth cones via regulation of TRPC1 channel opening. Acts also as a regulator of microtubule dynamics by inhibiting MAPT/TAU ability to promote microtubule assembly.

Protein type: Isomerase; EC 5.2.1.8; Chaperone

Chromosomal Location of Human Ortholog: 12p13.33

Cellular Component: nucleoplasm; microtubule; endoplasmic reticulum membrane; mitochondrion; cell soma; perinuclear region of cytoplasm; cytoplasm; cytosol

Molecular Function: protein binding, bridging; protein binding; peptidyl-prolyl cis-trans isomerase activity; FK506 binding; GTP binding; heat shock protein binding; phosphoprotein binding; tau protein binding; copper-dependent protein binding; ATP binding; glucocorticoid receptor binding

Biological Process: prostate gland development; male sex differentiation; steroid hormone receptor complex assembly; protein complex localization; protein peptidyl-prolyl isomerization; protein folding; negative regulation of microtubule polymerization; androgen receptor signaling pathway; negative regulation of microtubule polymerization or depolymerization; copper ion transport; embryo implantation