Rabbit anti-Human HEXIM1 Polyclonal Antibody | anti-HEXIM1 antibody

HEXIM1 (Protein HEXIM1, Cardiac Lineage Protein 1, Estrogen Down-regulated Gene 1 Protein, Hexamethylene Bis-acetamide-inducible Protein 1, Menage a Quatre Protein 1, CLP1, EDG1, HIS1, MAQ1) (Biotin)

Cat. Num. AAA6381003

• Gene Names: CLP1; HEAB; hClp1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: HEXIM1; Polyclonal Antibody; HEXIM1 (Protein HEXIM1; Cardiac Lineage Protein 1; Estrogen Down-regulated Gene 1 Protein; Hexamethylene Bis-acetamide-inducible Protein 1; Menage a Quatre Protein 1; CLP1; EDG1; HIS1; MAQ1) (Biotin); anti-HEXIM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CLP1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 425

• Applicable Applications for anti-HEXIM1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human CLP1, aa1-425 (NP_006822.1).
• Immunogen Sequence: MGEEANDDKKPTTKFELERETELRFEVEASQSVQLELLTGMAEIFGTELTRNKKFTFDAGAKVAVFTWHGCSVQLSGRTEVAYVSKDTPMLLYLNTHTALEQMRRQAEKEEERGPRVMVVGPTDVGKSTVCRLLLNYAVRLGRRPTYVELDVGQGSVSIPGTMGALYIERPADVEEGFSIQAPLVYHFGSTTPGTNIKLYNKITSRLADVFNQRCEVNRRASVSGCVINTCGWVKGSGYQALVHAASAFEVDVVVVLDQERLYNELKRDLPHFVRTVLLPKSGGVVERSKDFRRECRDERIREYFYGFRGCFYPHAFNVKFSDVKIYKVGAPTIPDSCLPLGMSQEDNQLKLVPVTPGRDMVHHLLSVSTAEGTEENLSETSVAGFIVVTSVDLEHQVFTVLSPAPRPLPKNFLLIMDIRFMDLK
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of CLP1 expression in transfected 293T cell line by CLP1 polyclonal antibody. Lane 1: CLP1 transfected lysate (47.6kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-HEXIM1 antibody

Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity.

Product Categories/Family for anti-HEXIM1 antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 5803029
• NCBI GeneID: 10978
• NCBI Accession #: NP_006822.1
• NCBI GenBank Nucleotide #: NM_006831.1
• UniProt Accession #: Q92989
• NCBI Official Full Name: polyribonucleotide 5'-hydroxyl-kinase Clp1 isoform 1
• NCBI Official Synonym Full Names: cleavage factor polyribonucleotide kinase subunit 1
• NCBI Official Symbol: CLP1
• NCBI Official Synonym Symbols: HEAB; hClp1
• NCBI Protein Information: polyribonucleotide 5'-hydroxyl-kinase Clp1
• UniProt Protein Name: Polyribonucleotide 5'-hydroxyl-kinase Clp1
• Protein Family: Protein
• UniProt Gene Name: CLP1

NCBI Description

This gene encodes a member of the Clp1 family. The encoded protein is a multifunctional kinase which is a component of the tRNA splicing endonuclease complex and a component of the pre-mRNA cleavage complex II. This protein is implicated in tRNA, mRNA, and siRNA maturation. Mutations in this gene are associated with pontocerebellar hypoplasia type 10 (PCH10). Alternatively splice transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

Uniprot Description

Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Plays a key role in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex: phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA (PubMed:24766809, PubMed:24766810). Its role in tRNA splicing and maturation is required for cerebellar development (PubMed:24766809, PubMed:24766810). Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.