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Testing Data (Staining of HUT78 cells with Mouse anti Human CD90: RPE (MBS213891))

Mouse CD90 Monoclonal Antibody | anti-CD90 antibody

MOUSE ANTI HUMAN CD90

Gene Names
THY1; CD90
Reactivity
Cynomolgus monkey
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation
Synonyms
CD90; Monoclonal Antibody; MOUSE ANTI HUMAN CD90; anti-CD90 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Cynomolgus monkey
Clonality
Monoclonal
Isotype
IgG1
Clone Number
F15-42-1
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
161
Applicable Applications for anti-CD90 antibody
Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections.
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Histology Positive Control Tissue
Human Brain, thymus
Buffer Solution
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of HUT78 cells with Mouse anti Human CD90: RPE (MBS213891))

Testing Data (Staining of HUT78 cells with Mouse anti Human CD90: RPE (MBS213891))

Testing Data

(Staining of HUT78 T cells with Mouse anti Human CD90:FITC (MBS211121))

Testing Data (Staining of HUT78 T cells with Mouse anti Human CD90:FITC (MBS211121))

Testing Data

(Staining of HUT78 T monocytes with Mouse anti Human CD90:Alexa Fluor 647 (MBS213193A647))

Testing Data (Staining of HUT78 T monocytes with Mouse anti Human CD90:Alexa Fluor 647 (MBS213193A647))
Related Product Information for anti-CD90 antibody
Mouse anti Human CD90 antibody, clone F15-42-1 recognizes the human CD90 cell surface antigen, a 25kD glycoprotein homologous to rat Thy1. The antigen is expressed by a subset of CD34+ve cells in the bone marrow and by prothymocytes within the thymus. CD90 is also expressed extensively within the brain. Mouse anti Human CD90 antibody, clone F15-42-1 is routinely tested in flow cytometry on the MOLT4 cell line.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,935 Da
NCBI Official Full Name
thy-1 membrane glycoprotein preproprotein
NCBI Official Synonym Full Names
Thy-1 cell surface antigen
NCBI Official Symbol
THY1
NCBI Official Synonym Symbols
CD90
NCBI Protein Information
thy-1 membrane glycoprotein; CDw90; Thy-1 T-cell antigen; thy-1 antigen
UniProt Protein Name
Thy-1 membrane glycoprotein
UniProt Gene Name
THY1
UniProt Entry Name
THY1_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

THY1: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Protein type: Membrane protein, GPI anchor; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: anchored to external side of plasma membrane; growth cone; focal adhesion; endoplasmic reticulum; integral to plasma membrane; dendrite; apical plasma membrane; plasma membrane; cytosol; lipid raft; external side of plasma membrane

Molecular Function: integrin binding; protein binding; protein kinase binding

Biological Process: focal adhesion formation; negative regulation of T cell receptor signaling pathway; cytoskeleton organization and biogenesis; T cell receptor signaling pathway; cell-cell adhesion; retinal cone cell development; negative regulation of protein kinase activity; negative regulation of axonogenesis; positive regulation of T cell activation; positive regulation of release of sequestered calcium ion into cytosol; angiogenesis; negative regulation of cell migration; positive regulation of GTPase activity

Research Articles on CD90

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Product Notes

The CD90 thy1 (Catalog #AAA213193) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN CD90 reacts with Cynomolgus monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CD90 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen*, Flow cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Immunohistology - Frozen: Application Note: The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. MyBioSource recommends the use of acetone fixation for frozen sections. Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50. Researchers should empirically determine the suitability of the CD90 thy1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD90, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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