Rabbit anti-Human ZHX2 Polyclonal Antibody | anti-ZHX2 antibody

ZHX2, NT (ZHX2, AFR1, KIAA0854, RAF, Zinc fingers and homeoboxes protein 2, Alpha-fetoprotein regulator 1, Regulator of AFP, Zinc finger and homeodomain protein 2) (APC)

Cat. Num. AAA6365316

• Gene Names: ZHX2; RAF; AFR1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ZHX2; Polyclonal Antibody; NT (ZHX2; AFR1; KIAA0854; RAF; Zinc fingers and homeoboxes protein 2; Alpha-fetoprotein regulator 1; Regulator of AFP; Zinc finger and homeodomain protein 2) (APC); Zinc finger and homeodomain protein 2; anti-ZHX2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-ZHX2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ZHX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 104-131 amino acids selected from the N-terminal region of human ZHX2.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(ZHX2 Antibody (N-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ZHX2 antibody detected the ZHX2 protein (arrow).)

Immunofluorescence (IF)

(Fluorescent confocal image of A549 cell stained with ZHX2 Antibody (N-term).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZHX2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). ZHX2 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.)

Related Product Information for anti-ZHX2 antibody

The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family.

Product Categories/Family for anti-ZHX2 antibody

Antibodies; Transcription Factors; Zinc; Ring; Homeobox

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Xu, J., et al. Proc. Natl. Acad. Sci. U.S.A. 107(5):2136-2140(2010). Trynka, G., et al. Gut 58(8):1078-1083(2009). Shen, H., et al. J. Cell. Mol. Med. 12 (6B), 2772-2780 (2008): Hu, S., et al. Neoplasma 54(3):207-211(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 7662342
• NCBI GeneID: 22882
• NCBI Accession #: NP_055758.1
• NCBI GenBank Nucleotide #: NM_014943.3
• UniProt Accession #: Q9Y6X8
• Molecular Weight: 92,307 Da
• NCBI Official Full Name: zinc fingers and homeoboxes protein 2
• NCBI Official Synonym Full Names: zinc fingers and homeoboxes 2
• NCBI Official Symbol: ZHX2
• NCBI Official Synonym Symbols: RAF; AFR1
• NCBI Protein Information: zinc fingers and homeoboxes protein 2; AFP regulator 1; regulator of AFP; transcription factor ZHX2; alpha-fetoprotein regulator 1; zinc-fingers and homeoboxes 2; zinc finger and homeodomain protein 2
• UniProt Protein Name: Zinc fingers and homeoboxes protein 2
• Protein Family: Zinc fingers and homeoboxes protein
• UniProt Gene Name: ZHX2
• UniProt Synonym Gene Names: AFR1; KIAA0854; RAF; AFP regulator 1
• UniProt Entry Name: ZHX2_HUMAN

NCBI Description

The members of the zinc fingers and homeoboxes gene family are nuclear homodimeric transcriptional repressors that interact with the A subunit of nuclear factor-Y (NF-YA) and contain two C2H2-type zinc fingers and five homeobox DNA-binding domains. This gene encodes member 2 of this gene family. In addition to forming homodimers, this protein heterodimerizes with member 1 of the zinc fingers and homeoboxes family. [provided by RefSeq, Jul 2008]