Mouse FHL2 Monoclonal Antibody | anti-FHL2 antibody

FHL2 (Four and a Half LIM Domains 2, DRAL, SLIM3)

Cat. Num. AAA608019

• Gene Names: FHL2; DRAL; AAG11; FHL-2; SLIM3; SLIM-3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified
• Synonyms: FHL2; Monoclonal Antibody; FHL2 (Four and a Half LIM Domains 2; DRAL; SLIM3); Anti -FHL2 (Four and a Half LIM Domains 2; anti-FHL2 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9G187
• Specificity: Recognizes the LIM-only protein FHL2. F4B2-B11 recognizes the N-terminal Zn-Finger motif, it does not crossreact in Western Blotting with the FHL1 and FHL3 proteins. Species crossreactivity: mouse, rat
• Purity/Purification: Affinity Purified; Purified
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.

• Applicable Applications for anti-FHL2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence (paraformaldehyde fixed cells), Western Blot, and Immunohistochemistry.; Dilution: Immunofluorescence (paraformaldehyde fixed cells): 1:10; Western Blot: 1:10; Immunohistochemistry (frozen and paraffin): 1:10
• Immunogen: Bacterially expressed mouse GST-FHL2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-FHL2 antibody

Five proteins share the same structural organization and a high degree of sequence homology in this group: four and a half LIM domain protein (FHL) 1, FHL2, FHL3, FHL4, and activator of cAMP-responsive element (CRE) modulator (CREM) in testis (ACT). LIM domains are constituted by a conserved cysteine- and histidine-rich structure shaped in two repeated zinc fingers first identified in the proteins encoded by the Lin-11, Isl-1, and Mec-3 genes. The LIM domain has been shown to function as a protein-protein interaction domain, and has often been described in association with other functional protein motifs, such as homeobox and kinase domains. FHL2 seems to be a promiscuous coactivator because it modulates the activity of the androgen receptor, CRE-binding protein (CREB), and WT1, although some degree of specificity is present because it is unable to stimulate CREM- and Sp1-dependent transcription. FHL2 expression was originally described to be restricted to the heart but it is inducible in other cell types. FHL2 shows

Product Categories/Family for anti-FHL2 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 87159836
• NCBI GeneID: 2274
• NCBI Accession #: NP_001034581.1
• NCBI GenBank Nucleotide #: NM_001039492.2
• UniProt Accession #: Q14192; Q13229; Q13644; Q9P294; Q2I5I4; Q2TSB7; Q2XQU9; Q53QP3; Q53T40; Q5TM15; Q6I9R8
• Molecular Weight: 32,193 Da
• NCBI Official Full Name: four and a half LIM domains protein 2
• NCBI Official Synonym Full Names: four and a half LIM domains 2
• NCBI Official Symbol: FHL2
• NCBI Official Synonym Symbols: DRAL; AAG11; FHL-2; SLIM3; SLIM-3
• NCBI Protein Information: four and a half LIM domains protein 2; OTTHUMP00000203164; OTTHUMP00000203165; OTTHUMP00000203166; OTTHUMP00000203167; OTTHUMP00000203168; OTTHUMP00000203169; OTTHUMP00000203172; LIM domain protein DRAL; aging-associated gene 11; skeletal muscle LIM-protein 3; four and a half LIM-domain protein 2; down-regulated in rhabdomyosarcoma LIM protein
• UniProt Protein Name: Four and a half LIM domains protein 2
• Protein Family: Four and a half LIM domains protein
• UniProt Gene Name: FHL2
• UniProt Synonym Gene Names: DRAL; SLIM3
• UniProt Entry Name: FHL2_HUMAN

NCBI Description

LIM proteins contain a highly conserved double zinc finger motif called the LIM domain.[supplied by OMIM]

Uniprot Description

FHL2: May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation.

Protein type: Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2q12.2

Cellular Component: nucleoplasm; focal adhesion; M band; nucleus; Z disc; actin cytoskeleton

Molecular Function: identical protein binding; protein binding; androgen receptor binding; zinc ion binding; transcription coactivator activity; transcription factor binding

Biological Process: osteoblast differentiation; atrial cardiac muscle cell development; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; ventricular cardiac muscle cell development; response to hormone stimulus; cellular lipid metabolic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of apoptosis