Rabbit FHL2 Polyclonal Antibody | anti-FHL2 antibody

FHL2 Polyclonal Antibody

Cat. Num. AAA2522036

• Gene Names: FHL2; DRAL; AAG11; FHL-2; SLIM3; SLIM-3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: FHL2; Polyclonal Antibody; FHL2 Polyclonal Antibody; DRAL; AAG11; FHL-2; SLIM3; SLIM-3; anti-FHL2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1.9mg/mL (varies by lot)
• Sequence Length: 279

• Applicable Applications for anti-FHL2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1000-1:5000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human FHL2
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Mouse heart tissue using FHL2 Polyclonal Antibody at dilution of 1:1900)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human cervical cancer using FHL2 Polyclonal Antibody at dilution of 1:40)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human brain using FHL2 Polyclonal Antibody at dilution of 1:40)

Related Product Information for anti-FHL2 antibody

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants, encoding the same protein, have been identified.

NCBI and Uniprot Product Information

• NCBI GI #: 87159836
• NCBI GeneID: 2274
• NCBI Accession #: NP_001034581.1
• NCBI GenBank Nucleotide #: NM_001039492.2
• Molecular Weight: 15,890 Da
• NCBI Official Full Name: four and a half LIM domains protein 2
• NCBI Official Synonym Full Names: four and a half LIM domains 2
• NCBI Official Symbol: FHL2
• NCBI Official Synonym Symbols: DRAL; AAG11; FHL-2; SLIM3; SLIM-3
• NCBI Protein Information: four and a half LIM domains protein 2; LIM domain protein DRAL; aging-associated gene 11; down-regulated in rhabdomyosarcoma LIM protein; skeletal muscle LIM-protein 3
• UniProt Protein Name: Four and a half LIM domains protein 2
• Protein Family: Four and a half LIM domains protein
• UniProt Gene Name: FHL2
• UniProt Synonym Gene Names: DRAL; SLIM3; FHL-2; SLIM-3
• UniProt Entry Name: FHL2_HUMAN

NCBI Description

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

FHL2: May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation.

Protein type: Motility/polarity/chemotaxis; Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 2q12.2

Cellular Component: nucleoplasm; focal adhesion; M band; nucleus; Z disc; actin cytoskeleton

Molecular Function: identical protein binding; protein binding; androgen receptor binding; zinc ion binding; transcription coactivator activity; transcription factor binding

Biological Process: osteoblast differentiation; atrial cardiac muscle cell development; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; ventricular cardiac muscle cell development; androgen receptor signaling pathway; response to hormone stimulus; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; negative regulation of apoptosis